组织工程皮肤与正常皮肤组织学差异的实验研究  

Experimental research of histological difference between tissue-engineered skin and normal human skin

作　　者：李媛[1] 陆伟[1,2] 冀堃[2] 刘源[1] 董绍忠[1] 金岩[1,2] 

机构地区：[1]第四军医大学口腔医学院口腔组织病理学教研室 [2]第四军医大学组织工程研发中心,陕西西安710033

出　　处：《第四军医大学学报》2009年第24期2921-2924,共4页Journal of the Fourth Military Medical University

基　　金：国家高技术研究发展计划(863计划)组织工程皮肤重大专项(2006AA02A119);陕西省社发攻关项目(2009K14-03)

摘　　要：目的:研究组织工程皮肤与正常皮肤表皮层组织学的差异,并探讨其意义.方法:用组织工程方法构建人全层皮肤,以正常在体皮肤作为对照,组织学、免疫细胞化学、透射电镜观察表皮层的异同.结果:组织工程皮肤与正常皮肤在组织学上有一定差异,缺少上皮钉突,CK19在组织工程皮肤成熟期表达阴性,但CK10和pan-CK在两者表达皆为阳性,透射电镜观察上皮结构接近正常皮肤.结论:组织工程皮肤表皮层与真皮层结合不如正常皮肤紧密,可能与缺少上皮钉突有关,其表皮细胞基本分化成熟.AIM：To study the differences in histology of the tissue-engineered skin and normal human skin.METHODS：The bilayer skin replacement was constructed by tissue engineering method,compared with the normal human skin by histological and immunohistochemistry methods.RESULTS：There were some differences in tissue-engineered skin and the normal skin.Epidermis rete pegs were presented only in the normal skin,CK19 was negative in the matured tissue engineered-skin,but both CK10 and pan-CK were positively expressed in tissue-engineered skin and the normal skin.Epithelium structure by TEM was similar to the normal skin.CONCLUSION：The dermo-epidermal junction in tissue-engineered skin was less tighter than normal skin,may be due to the absence of epidermis rete pegs,epidermal cells had differentiated.

关 键 词：组织工程皮肤 正常皮肤 再生 

分 类 号：R318.19[医药卫生—生物医学工程]