兔VX2肝癌模型不同生长期的CT灌注成像与组织学对照研究  

Control study of CT perfusion imaging and histology of rabbit liver VX2 tumor in different growth period

作　　者：吴越[1] 梁长虹[1] 刘昕[2] 杨本强[3] 刘文源[3] 李子浩[3] 

机构地区：[1]广东省人民医院放射科,广东广州510080 [2]沈阳军区总医院整形外科,辽宁沈阳110016 [3]沈阳军区总医院放射诊断外科,辽宁沈阳110016

出　　处：《第四军医大学学报》2009年第24期3009-3013,共5页Journal of the Fourth Military Medical University

摘　　要：目的:通过对兔VX2肝癌模型不同生长期的CT灌注参数与组织学进行研究,为CT灌注成像成为评价该肿瘤生长的影像学手段提供实验依据.方法:16只新西兰大白兔,采用开腹瘤块包埋法建立兔VX2肝癌模型,分别于种瘤前、种瘤后第1周到第3周行多层螺旋CT灌注扫描,对比种瘤前肝实质与种瘤后肿瘤区域以及第2周肿瘤区与非肿瘤区肝脏的肝血流量(HBF)、肝血容积(HBV)、平均通过时间(MTT)、毛细血管表面通透性(PS)和肝动脉分数(HAF)的变化规律.种瘤后第3周CT检查完成后处死实验兔,进行病理与血管内皮生长因子(VEGF)免疫组织化学染色.所有结果均进行统计学分析.结果:16只兔均接种成功.肿瘤部位种瘤后1～3wkHBF,PS,HAF值与种瘤前比较差异有显著性意义(P<0.01),HBV,MTT值差异无显著性意义.种瘤后第2周肿瘤区HBF,HBV,PS,HAF增高与非肿瘤区肝组织比较有统计学差异(P<0.01).肿瘤区MTT降低与非肿瘤区肝组织比较有统计学差异(P<0.01).大体病理检查共发现16个单发肿瘤,其中14例中心出现坏死囊变区,与CT检查基本一致.光学低倍镜下见肿瘤细胞在肝内呈浸润性癌巢,与肝实质无明显边界,新生毛细血管丰富;高倍镜下可见瘤细胞体积大,呈不规则排列,核分裂像多见.VEGF染色阳性呈棕褐色,多见于细胞质内,呈均匀或不均匀的颗粒状或团块状;随着癌细胞增生活跃,阳性细胞数量增多,染色也增强.结论:多层螺旋CT灌注成像能较好地评价兔VX2肝癌模型的血流动力学变化,结合了组织学的研究则可以提供更多的量化信息.AIM：To study the computed tomography perfusion parameter change and histology of rabbit liver VX2 tumor in different growth period,it was supposed to provide experimental basis for evaluation of computed tomography perfusion imaging in measurement of effect assessment of liver tumor.METHODS：The multi-slice computed tomography perfusion was performed in 16 New Zealand white rabbits at before and 1 to 3 weeks after VX2 hepatic tumor implantation.The various perfusion parameters（HBF,HBV,MTT,PS,HAF） were measured in the tumor area and non-tumorous regions and different growth period.After that,all the rabbits were executed and pathological examination and VEGF staining was performed.All of statistical data were compared respectively.RESULTS：Sixteen（100%）rabbits were successfully implanted with VX2 tumor in the liver.It was demonstrated that significant difference between HBF,PS,HAF of liver prior to tumor implantation and 1 to 3 weeks after implantation（P0.01）.And no significant difference in HBV,MTT.The statistical analysis also showed the increased HBF,HBV,PS,HAF and the decreased MTT in the tumor area were differed from the non-tumorous regions at 2 weeks after VX2 hepatic tumor implantation（P0.01）.Sixteen single tumor were detected in pathology,the central parts of tumors in the group of fourteen showed the cystic necrosis according to the result of CT imaging.The light microscopic observation of low magnification showed that the cells of tumor were arranged into nests with fibrous tissue in the liver,there was no clear margin between tumor and liver parenchyma,and abundant capillary of developed was been seen also;high magnification showed that the cells of tumor were bigger than the normal liver cells,cells distributed irregularly with abundant caryokinesis.VEGF positive staining could be seen most in cytoplasm as uniform or uniform granule,clot in brown-yellow color;with the cells of tumor proliferated more actively,positive cells were more abundant,and staining more stronger.CONCLUSION�

关 键 词：体层摄影术 VX2肿瘤 灌注成像 

分 类 号：R814.42[医药卫生—影像医学与核医学]