DACH1蛋白在甲状腺乳头状癌中的表达及其意义  

Immunohistochemic expression of DACH1 and its clinical significance in papillary thyroid carcinoma

作　　者：赵昕[1] 周洁[1] 司翠[1] 王文勇[2] 张南雁[1] 王晨光 姬秋和[1] 

机构地区：[1]第四军医大学西京医院内分泌代谢科 [2]第四军医大学基础部病理学教研室,陕西西安710033 [3]美国托马斯杰佛逊大学

出　　处：《第四军医大学学报》2009年第24期3055-3057,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨DACH1的表达与甲状腺乳头状癌的发生、发展及预后的关系.方法:收集临床病理标本40例,其中甲状腺乳头状癌30例、结节性甲状腺肿10例.运用免疫组织化学SABC法,以多克隆抗体兔抗人DACH1进行免疫组织化学染色,镜下观察不同甲状腺组织中DACH1的表达,比较其阳性率,分析其与临床病理特性的相关性.结果:DACH1蛋白在甲状腺乳头状癌中表达阳性率高达76.7%(23/30),其中强阳性约46.6%(14/30).而DACH1在结节性甲状腺肿中的表达仅为30%(3/10),且仅为弱阳性.统计分析表明具有显著性差异(P<0.01).而且DACH1蛋白的表达与甲状腺乳头状癌有否淋巴结转移、有无包膜外侵犯有关(P<0.05),而与年龄、肿瘤大小、临床分期无关(P>0.05).结论:DACH1的异常表达可能参与甲状腺乳头状癌的发生发展,在其发病中起重要作用.AIM：To explore the immunohistochemical expression of DACH1 in papillary thyroid carcinoma and its clinical significance.METHODS：DACH1 expression in 10 nodular goiters,and 30 papillary thyroid carcinomas were detected with SABC immunohistochemical method,and the relationship between DACH1 expression and clinicopathological characteristics were further analyzed.RESULTS：The immunohistochemic expression of DACH1 in papillary thyroid carcinoma and goiter is orientated as the cytomembrane and cytoplasm.The positive rate of DACH1 is 76.7% in papillary thyroid carcinoma,notably higher than that in nodular goiter,later is 30%（P0.01）.The positive rate of expression of DACH1 protein in papillary thyroid carcinoma with lymph nodes metastasis was 89.5%,while it was 54.5% in group of without lymph nodes metastasis.The differences are significant（P0.05）.CONCLUSION：The DACH1 protein is immunohistochemic overpressed in papillary thyroid carcinoma.There is obvious relationship between the degree of infiltration and metastasis of lymph node,indicating that DACH1 may play a role in the carcinogenesis and development of papillary thyroid carcinoma.

关 键 词：DACH1蛋白 甲状腺乳头状癌 免疫组织化学 

分 类 号：R581[医药卫生—内分泌]