瑞舒伐他汀对大鼠冠状动脉微栓塞后心肌局部炎症反应及左心室功能的影响  

Effect of Rosuvastatin on Myocardial Inflammation and Left Ventricular Disfunction After Coronary Microembolization in Rats

作　　者：傅发源[1] 张飞龙[1] 陈良龙[1] 

机构地区：[1]福建医科大学附属协和医院心内科,福建省福州市350001

出　　处：《中国动脉硬化杂志》2009年第11期907-911,共5页Chinese Journal of Arteriosclerosis

基　　金：福建省卫生厅青年基金课题(2004-1-17)

摘　　要：目的探讨围手术期瑞舒伐他汀干预对冠状动脉微栓塞后心肌局部炎症反应及左心室功能的影响。方法雄性SD大鼠48只,随机分为对照组、微栓塞组、瑞舒伐他汀低剂量组[0.5 mg/(kg.d)]和高剂量组[3.0mg/(kg.d)],每组分为术后3天、28天两个观察时间点,每时间点6只,瑞舒伐他汀干预的时间从术前7天到术后7天。术后3天,检测炎症因子肿瘤坏死因子α、白细胞介素1β、白细胞介素10的表达,观察心肌组织病理变化;术后28天,检测心肌组织胶原纤维含量的变化,观察左心室功能改变。结果与微栓塞组比较,瑞舒伐他汀高剂量组能明显抑制心肌细胞肿瘤坏死因子α、白细胞介素1β表达,增加白细胞介素10表达;同时减少心肌组织微梗死灶数量,减轻炎症细胞浸润,减少心肌胶原纤维增生,并改善左心室功能。而低剂量组则不能。结论围术期高剂量瑞舒伐他汀干预能抑制大鼠冠状动脉微栓塞后心肌局部炎症反应,减轻心脏重塑,并改善左心室功能。Aim To evaluate the effect of rosuvastatin on myocardial inflammation and left ventricular disfunction after coronary microembolization（CME）. Methods 48 male SD rats were randomized into control group,CME group,low-dose rosuvastatin group[0.5 mg/（kg·d）] and high-dose rosuvastatin group [3.0 mg/（kg·d）] averagely.Rosuvastatin was fed from pre-to post-CME for two weeks.Αt 3 days post-CME,the expression of tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β）,interleukin-10（IL-10） in myocardium was assayed by ELISΑ,immunostaining and pathological changes were detected by HE staining.Αt 28 day post-CME,collagen deposition was detected by Masson staining and cardiac function was assessed by echocardiography. Results The levels of TNF-αand IL-1β were lower,and IL-10 was higher in high-dose rosuvastatin group compared with CME group.High-dose but not low-dose rosuvastatin also reduced inflammatory cell infiltration and collagen deposition in farct zone,decreased LVEDD,LVESD and increased LVEF,LVFS. Conclusion In rats with CME,perioperative therapy with high-dose rosuvastatin prevents cardiac remodeling and dysfunction.This benefit may be partly derived from reducing myocardial inflammation.

关 键 词：瑞舒伐他汀 冠状动脉微栓塞 炎症反应 左心室功能 

分 类 号：R5[医药卫生—内科学]