HPLC法测定汝安膜中盐酸小檗碱含量  

Determination of berberine hydrochloride contents of RU AN membrane by HPLC

作　　者：郭新社[1] 李武营[2] 

机构地区：[1]河南大学药学院药物分析教研室,河南开封475001 [2]河南大学学报编辑部,河南开封475001

出　　处：《河南大学学报（医学版）》2009年第4期281-283,共3页Journal of Henan University:Medical Science

基　　金：开封市科技攻关项目(030003)

摘　　要：目的：建立汝安膜中盐酸小檗碱的含量测定方法。方法：使用C18柱,0.03 mol/L磷酸二氢钠溶液-乙腈（68∶32）为流动相,检测波长为348 nm。结果：盐酸小檗碱在0.4-2.0 mg/L范围内线性关系良好,平均回收率为98.04%（n=6）,RSD为0.89%。结论：该方法准确、可靠、重现性好,可作为该膜剂的质量控制方法。Objective： To establish the method of detecting berberine hydrochloride contents of.Methods： HPLC was used to determine berberine hydrochloride in RU AN membrane on C18 column,using 0.03 mol/L NaH2PO4-CH3CN（68∶32） as mobile phase,with detection at 348 nm.Results： The good linearity was observed for the concentration of 0.4-2.0 mg/L.The average recoveries were 98.04%（n = 6）.RSD were 0.89%.Conclusion： It is accurate,safe,good in reappearing rate,and can be used as the quality control method for the membrane medication.

关 键 词：汝安膜 盐酸小檗碱 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]