PVL基因阳性与阴性MRSA对坏死性肺炎的致病性比较及机制  

Comparison of the pathogenicity between PVL gene(+) MRSA and PVL gene(-) ones in necrotizing pneumonia

作　　者：吕小艳[1] 马筱玲[2] 戴媛媛[2] 张义永[2] 荚恒敏[2] 高玉录[2] 付广林[1] 

机构地区：[1]蚌埠医学院,安徽蚌埠233000 [2]安徽医科大学附属省立医院

出　　处：《山东医药》2010年第3期10-12,共3页Shandong Medical Journal

基　　金：安徽高校省级自然科学基金资助项目(KJ2008B065)

摘　　要：目的研究Panton-Valentine杀白细胞素(PVL)基因对耐甲氧西林金黄色葡萄球菌(MRSA)在坏死性肺炎中致病能力的影响及机制。方法取18只小鼠随机分为模型1组、模型2组及对照组各6只,分别通过气管灌注MRSA标准菌株ATCC43300、铜-23菌悬液及生理盐水,实验后24 h及48 h分别检测三组外周血白细胞计数。实验后48 h光镜观察肺组织病理结构,收集支气管肺泡灌洗液(BALF)脱落细胞以电镜观察形态变化、流式细胞术检测凋亡率和坏死率。结果与对照组比较,实验后24 h和48 h外周血白细胞计数模型2组下降、模型1组升高;模型2组肺组织炎细胞浸润及支气管上皮细胞破坏情况重于模型1组、BALF中脱落细胞坏死率高于模型1组。结论PVL基因阳性CA-MRSA对坏死性肺炎的致病性强于PVL基因阴性MRSA,可能机制为前者能够分泌PVL基因并可编码PVL毒素。Objective To investigate the effect of Panton-Valentine leukocidin （PVL） expression on the pathogenicity of methieillin-resistant staphylococcus aureus（MRSA） in necrotizing pneumonia. Methods Eighteen mice were randomly distributed into model 1, model 2 and normal group, then were intratracheally instilled by Staphylococcus aureus ATCCA3300, Cu-23 bacterial suspension and physiological saline respectively. The WBC in peripheral blood of the three groups were counted respectively 24 and 48 hours after instilling, pathologic examinations of the lung histology were performed by optical microscope. The bronehoalveolar lavage fluid （BALF） were collected and the morphological changes of exfoliated eeUs were observed by electron microscopy （TEM）, the apoptosis and necrosis rates were analyzed by flow eytometry. Results Compared with the control group, the WBC count of model 2 group decreased 24 and 48 hours after instilling,but whleh in model 1 group increased. The inflammatory infiltration in lung parenehyma and bronchial epithelial damage in model 2 group were more serious than which in model 1 group. Necrosis rates of exfoliated cells in BALF were higher in model 2 group than which in model 1 group. Conclusions The pathogenicity of PVL gene （ ＋ ） MRSA is greater than that of the PVL gene（ - ）MRSA,the mechanism may be that the former can encode PVL.

关 键 词：耐甲氧西林金黄色葡萄球菌 Panton-Valentine杀白细胞素 坏死性肺炎 社区获得性感染 

分 类 号：R563.1[医药卫生—呼吸系统]