轮状病毒肠道外感染乳鼠模型的建立  

Establishment of suckling mouse model of extraintestinal rotavirus infection

作　　者：夏群[1] 陈兰举[1] 陈名武[1] 周瑞[1] 沈怀云[1] 丁周志[1] 徐家丽[1] 薛莲[1] 刘欣[1] 

机构地区：[1]蚌埠医学院第一附属医院儿科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2010年第1期1-3,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助项目(2006kj107C)

摘　　要：目的：建立轮状病毒（rotavirus,RV）肠道外感染乳鼠模型。方法：实验动物选用10-11日龄的清洁级ICR乳鼠,将32只乳鼠随机分为2组,每组16只,即RV肠道外感染组：乳鼠经腹腔注射150μl猴RV SA l1（病毒滴度TC ID50为10-4）;对照组：乳鼠经腹腔注射150μl生理盐水。接种后每天观察乳鼠的活动状况、饮食情况、毛色、体型、大便变化等,4天后处死,留取心、肝、肾、脾、肠进行病理学切片和匀浆培养。结果：感染组在接种RV后第2天开始出现食欲不振,体毛蓬松、无光泽,活动减少,呆滞、嗜睡,对刺激反应淡漠,大便量少,上述症状逐渐加重,到第4天症状最明显,并有3只乳鼠出现腹泻,观察中累积腹泻率和发病率分别为18.75%和100.00%。光镜下肝、肾、脾、小肠有病理改变,且肝、肾、小肠组织匀浆培养72 h后出现不同程度细胞病变,余阴性。对照组乳鼠无明显变化。结论：该方法初步建立了RV肠道外感染乳鼠模型。Objective：To establish the suckling mouse model of extraintestinal rotavirus infection.Methods：Thirty-two ICR mouse aged from 10 to 11 days were randomly divided into two groups,with 16 in each.The extraintestinal infection group were inoculated by intraperitoneal injection of 150 μl simian rotavirus SAl1（the virus titer of TCID50 was about 10^-4） and the control group were inoculated by intraperitoneal injection of 150 μl normal saline.After inoculation,the mouse activities,diet,somatotype,change of hair color and stool were observed.All the suckling mouse were killed 4 days later,and the heart,liver,kidney,spleen and intestines were collected for pathological section and homogenate culture.Results：After two days of infection,the suckling mouse in extraintestinal infection group began to lose appetite,their clothing hair was tarnished and activities were decreased;They were dull,lethargy and slow to outside stimulus;meanwhile,their stool amount was reduced;the symptoms aggravated gradually and reached the peak at the fourth day;moreover,three mouse demonstrated the symptom of diarrhea.In the whole process of observation,the cumulative diarrhea rate and inception rate were 18.75% and 100.00%,respectively.Pathological changes were observed in the liver,kidney,spleen and intestines by light-microscope.After 72 hours of homogenate culture,cytopathic effect was observed in the liver,kidney and intestines,but no obvious changes were noticed in the heart or spleen.The suckling mouse in control group presented normal condition.Conclusions：It is feasible to establish the suckling mouse model of extraintestinal rotavirus infection with this method.

关 键 词：轮状病毒 肠道外感染 乳鼠 动物模型 

分 类 号：R446.5[医药卫生—诊断学] R512.5[医药卫生—临床医学]