用逆转录-聚合酶链反应检测海南岛登革热流行区某些动物体内登革病毒RNA  

Detection of Dengue virus genome RNA in some kinds of animals caught from Dengue fever endemic areas in Hainan Island with reverse transcription-polymerase chain reaction

作　　者：张浩燕[1,2,2] 杨新科[1,2,2] 李桂英[1,2,2] 王凤莲 佟玉品[1,2,2] 

机构地区：[1]北京热带医学研究所 [2]中国预防医学科学院病毒学研究所

出　　处：《中华实验和临床病毒学杂志》1998年第3期226-228,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的寻找中国海南岛一带登革热疫区，登革病毒潜伏的动物宿主及鉴定其毒株型别。方法采用１－４型登革病毒通用引物，用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测海南岛登革热流行区蝙蝠脑细胞，血清和埃及伊蚊的登革病毒ＲＮＡ。结果检测３５例疫区蝙蝠脑细胞，２０例阳性；检测１８例蝙蝠血清，３例阳性；检测三组埃及伊蚊，１组阳性，３组非流行区者都阴性。用４个登革病毒原型株的单克隆荧光抗体技术检测２０例登革病毒ＲＮＡ阳性的蝙蝠脑细胞压印片，１６例为２型株阳性，与ＲＴ－ＰＣＲ检测登革热流行区登革病毒ＲＮＡ阳性蝙蝠脑细胞的阳性符合率为８０００％。用ＲＴ－ＰＣＲ检测非流行区蝙蝠脑细胞登革病毒ＲＮＡ，均为阴性。结论本研究证实蝙蝠是登革病毒的贮存宿主，为登革热流行的有效控制提供了一定重要的线索。Detection of dengue virus genome RNA in brain of bats caught from dengue fever endemic areas in Hainan Island were carried out with reverse transcription-polymerase chain reaction(RT-PCR). Positive result was demonstrated in 20 of 35 bats tested, with a positive rate of 57 14%; RT-PCR was applied for the detection of dengue virus genome RNA in sera of bats caught from the endemic areas in Hainan Island, 3(16 66%)of 18 sera were positive. Dengue virus genome RNA in female Aedes aegypti captured from dengue fever endemic areas in Hainan Island was detected by using RT-PCR, 1(33 33%) of 3 lots was positive. Similar examinations on bat brains and mosquitoes captured from non-endemic areas were all negative. In addition, monoclonal IgG antibodies against types 1-4 dengue viruses were added onto brain imprints of bats captured from endemic areas of dengue fever in Hainan Island, 16(80 00%) of 20 showed positive results for type 2 dengue virus antigen by direct immunofluorescent assay. The antibodies to various types of dengue virus were coincided to that of dengue virus genome RNA. The above results proved that bat is the reservoir of dengue virus, and this provides an important clue for the effective control of dengue fever epidemics in endemic areas.

关 键 词：聚合酶链反应 登革病毒 蝙蝠 RT-PCR 

分 类 号：R446.5[医药卫生—诊断学]