乙型肝炎病毒感染者癌基因蛋白的表达及与HBeAg的关系  

The relationship between expression of oncogene proteins and HBeAg in chronic hepatitis B virus infection

作　　者：洪丰 张春运 赵洪涛 石男[1,2,3,4] 

机构地区：[1]山东省济宁第一人民医院 [2]济宁市煤化医院 [3]山东省立医院肝病中心 [4]山东省医科大学病理检测中心

出　　处：《中华实验和临床病毒学杂志》1998年第3期249-250,共2页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的为了阐明癌基因蛋白异常表达与乙型肝炎病毒（ＨＢＶ）复制的关系。方法用链霉菌素－生物素（ＳＬＡＢ）免疫组织化学染色和酶联免疫吸附试验（ＥＬＩＳＡ）方法，检测了６４例慢性ＨＢＶ感染者肝细胞中Ｃ－ｅｒｂＢ－２Ｐ１８５、ｒａｓＰ２１和Ｐ５３等癌基因蛋白和血清乙型肝炎病毒ｅ抗原（ＨＢｅＡｇ）。结果Ｃ－ｅｒｂＢ－２Ｐ１８５和ｒａｓＰ２１阳性组中，血清ＨＢｅＡｇ的阳性检出率分别为８９４％和８４６％，而阴性组ＨＢｅＡｇ的检出率分别为２０％和４８％，两组差异显著。结论表明癌基因蛋白Ｃ－ｅｒｂＢ－２Ｐ１８５和ｒａｓＰ２１的异常表达与ＨＢＶ复制密切相关。To study the relationship between abnorma expression of oncogene protein and HBV replication, we detected the expression of protein products C-erbB-2 P185, ras P21 and tumorsuppressor gene product P53 and HBeAg in the serum of 64 patients with chronic HBV infection by using immunohistochemical technique and ELISA. The results were as follows. The positive detection rates of serum HBeAg in C-erbB-2 P185 and ras P21 oncogenes positive groups were 89 4% and 84 6% respectively, but only 20% and 48% in C-erbB-2 P185 and ras P21 negative groups. It suggested that there is a close correlation between the over-expression of C-erbB-2 P185, ras P21 and replication of HBV.

关 键 词：癌基因 乙型肝炎 HBEAG 

分 类 号：R512.62[医药卫生—内科学] R730.3[医药卫生—临床医学]