高压氧对新生大鼠缺氧缺血性脑病脑组织匀浆中SOD、GSH-PX及MDA的影响  

Effects of Hyperbaric Oxygenation on Newborn Rats with Hypoxicischemic Encephalopathy

作　　者：张丽达[1] 刘家浩[1] 姜素敏 杨胜敏 梁方志 田小香 

机构地区：[1]南京铁道医学院附属医院高压氧室

出　　处：《南京铁道医学院学报》1998年第3期156-158,共3页Journal of Nanjing Railway Medical College

基　　金：江苏省应用基础基金

摘　　要：目的：探讨高压氧（ＨＢＯ）对新生大鼠缺氧缺血性脑病（ＨＩＥ）的影响。方法：将７日龄ＳＤ新生大鼠随机分为对照组、ＨＩＥ组和ＨＢＯ治疗组。在实验第７天后处死动物。测定病侧脑组织匀浆超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）和丙二醛（ＭＤＡ）含量。结果：（１）ＨＩＥ模型组ＳＯＤ、ＧＳＨ－ＰＸ活力明显低于对照组（Ｐ＜０．０００３，Ｐ＜０．００６），而ＭＤＡ含量显著高于对照组（Ｐ＜０．００３）；（２）ＨＩＥ组ＨＢＯ治疗１周后，脑组织中ＳＯＤ、ＧＳＨ－ＰＸ活力明显高于未治疗ＨＩＥ组（Ｐ＜０．０００３，Ｐ＜０．００６），而ＭＤＡ含量低于未治疗ＨＩＥ组（Ｐ＜０．００３）。结论：ＨＢＯ能增加ＨＩＥ脑组织内自由基清除酶类的活力。Objective:To study the effects of hyperbaric oxygen(HBO) on newborn SD rats with hypoxicischemic encephalopathy(HIE).Methods:The newborn rats were randomized into control group,HIE group and HIE treated with HBO group.The rats were then killed and contents of SOD,GSH-PX and MDA in cerebral homogenate were measured.Results:(1)SOD,GSH-PX activity of HIE were lower than that of control group( P <0.003);(2)SOD,GSH-PX activity in HIE rats treated by HBO became higher than of HIE,MDA value was lower.Conclusion:HBO can decrease the production of the oxygen derived free radicals in brain after HIE.

关 键 词：高压氧 缺氧缺血性脑病 氧自由基 大鼠 

分 类 号：R722.12[医药卫生—儿科]