维生素D_3和维甲酸合并使用抑制HCE16/3细胞病毒早期基因表达的研究  

Vitamin D 3 Inhibits the Expression of Viral Early Genes in HCE16/3 Cells in Combination With Retinoic Acid

作　　者：郑杰[1] 

机构地区：[1]南京铁道医学院病理学教研室

出　　处：《南京铁道医学院学报》1998年第3期170-172,共3页Journal of Nanjing Railway Medical College

摘　　要：目的：研究维生素Ｄ３对人乳头瘤病毒（ＨＰＶ）１６型ＤＮＡ－永生化的人宫颈上皮细胞（ＨＣＥ１６／３细胞）病毒基因表达的调节。方法：使用Ｎｏｒｔｈｅｒｎ印迹法分析了维生素Ｄ３或（和）维甲酸处理的ＨＣＥ１６／３细胞病毒早期基因表达。结果：在缺乏血清和生长因子的培养条件下，单独使用维生素Ｄ３或维甲酸并不明显改变病毒早期基因的表达，但维生素Ｄ３加维甲酸合并使用可以抑制细胞病毒早期基因的表达。结论：维生素Ｄ３加维甲酸合并使用可以抑制细胞病毒早期基因的表达。Objective:To study the effect of the active metabolism of vitamin D, i.e., 1,25-dihydroxycholecalciferol (1,25-D 3) on the expression of viral early genes of human papillomavirus (HPV) type 16 DNA-immortalized human cervical epithelial cells (HCE16/3 cells).Mothods:The effect of 1,25-D 3 and retinoic acid, alone and in combination, on the expression of viral early genes in cultured HCE16/3 cells was determined by Northern blot.Results:Vitamin D 3 or retinoic acid alone had little effect on the expression of viral early genes of HCE16/3 cells. However, the expression of viral early genes in HCE16/3 cells was inhibited with vitamin D 3 in combination with retinoic acid.Conclusion:Vitamin D 3 inhibits the expression of viral early genes in HPV DNA-immortalized human cervical epithelial cells in combination with retinoic acid. It may provide the experimental base for treating HPV-associated tumors with vitamin D 3 and retinoic acid in the future.

关 键 词：维生素D3 维甲酸 病毒致瘤基因 

分 类 号：R977.24[医药卫生—药品] R977.21[医药卫生—药学]