27个菜心品种遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of 27 Flowering Chinese Cabbage

作　　者：孙雪梅[1,2] 乔爱民[1] 孙敏[2] 桂腾琴[1,2] 尹彩霞[1] 

机构地区：[1]仲恺农业工程学院农业与园林学院,广州510225 [2]西南大学生命科学学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2010年第1期119-123,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：广东省自然科学基金资助项目(B1010412)

摘　　要：利用ISSR技术对27个菜心品种的遗传多样性进行了分析.结果表明,12条引物共检测到103个可重复位点,其中多态位点58个,多态位点百分率(P)为56.31%.平均多样性指数(I)为0.229,遗传相似系数(H)为0.144,观察等位基因数(Na)和有效等位基因数(Ne)分别为1.563和1.225,遗传距离(GD)的范围在0.029～0.344之间,表明菜心遗传多样性较低.经UPGMA聚类分析,将27个菜心品种分为六大类群.Genetic diversity of 27 flowering Chinese cabbage （Brassica campestris L. ssp. chinensis var. utilis Tssen. et Lee） cultivars were analyzed with inter-- simple sequence repeat （ISSR） molecular mark ers. A total of 103 loci were detected using 12 ISSR primers, of which 58 were polymorphic, the percentage of polymorphic loci （p） being 56.31%. Shannon＇s information index （I） and Nei＇s gene diversity （H） were 0. 229 and 0. 144, respectively, observed number of alleles （Na） and effective number of alleles （Ne） were 1. 563 and 1. 225, respectively, and the genetic distance ranged from 0. 029 to 0. 344, indicating that the genetic diversity of flowering Chinese cabbage was relatively low. UPGMA cluster analysis clustered the 27 cultivars into six groups.

关 键 词：菜心 遗传多样性 ISSR 

分 类 号：Q789[生物学—分子生物学]