外源氮对离体麦穗干物质及氮素积累的影响  

Effects of N on Dry Matter and N Accumulation of Detached Wheat Spikes In Vitro Culture

作　　者：姜丽娜[1] 吕远[1] 侯飞[1] 冯俊利[1] 唐瑞霞[1] 栗志英[1] 祁诗月[1] 李春喜[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南农业科学》2010年第1期11-16,共6页Journal of Henan Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2006BAD02A13-2-3);河南省科技厅自然科学基金项目(082300430120);河南省高校青年骨干教师资助计划

摘　　要：采用离体穗培养的方法,选取花后7d的周麦18为材料,研究了外源氮(0、0.5、1.0、2.0 g/LNH4NO3)对离体麦穗干物质及氮素积累的影响。结果表明,随培养天数的增加,离体穗干物质积累总量呈增加趋势,外源氮促进了离体穗干物质的积累,提高了干物质在籽粒中的分配比例。在外源氮供给下,离体穗营养器官干物质向籽粒的转移率和贡献率均降低,籽粒光合产物积累量增加。外源氮增加了籽粒中氮素的含量和积累量,提高了离体穗中氮素在籽粒中的分配比例。在本试验中,2.0 g/L NH4NO3处理效果最佳,促进了离体穗尤其是籽粒的生长,提高了籽粒的氮素含量和积累量,对籽粒建成具有促进作用。Taking Zhoumai 18 as experiment material,the effects of N on dry matter and N accumulation of detached wheat spikes in vitro culture were studied under different levels of N application （0,0.5,1 and 2 g/L NH4NO3 ,respectively）. The spikes were collected 7 days after anthesis. The results showed that the dry matter accumulation of detached spikes increased with the increase of culture time. Exogenous N promoted the dry matter accumulation and improved the dis： tribution rate in grains in detached spikes. The supply of exogenous N decreased the translocation efficiency of dry matters from vegetative organs and their contribution rate to grain,but the photosynthetic product in grains increased. Exogenous N also improved the N accumulation in detached spikes,increased the N content and accumulation in grains, and promoted the N distribution rate in grains. In the study,the best treatment was 2.0g/L NH4NO3.

关 键 词：小麦 离体穗培养 氮素 干物质 

分 类 号：S512.1[农业科学—作物学]