棉花黄萎病拮抗菌株DS45-2的分离鉴定  

Isolation and Identification of Antagonistic Strain DS45-2 against Cotton Verticillium Wilt

作　　者：陈妍[1] 李红亚[1] 李术娜[1] 王全[1] 王伟[1] 王树香[1] 朱宝成[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河南农业科学》2010年第1期68-72,共5页Journal of Henan Agricultural Sciences

基　　金：河北省自然科学基金项目(398152)

摘　　要：从土样中筛选对棉花黄萎病致病菌大丽轮枝菌(Verticillium dahliaeKleb)具有拮抗作用的芽孢杆菌菌株,初筛得到具有拮抗作用的菌株68株,复筛选出1株拮抗活性较高的菌株DS45-2,通过形态特征观察,生理生化试验及16S rDNA序列分析对此菌株进行鉴定,初步鉴定此菌株属于解淀粉芽孢杆菌(Bacillus amyloliquefaciens),与相应标准菌株NBRC 15535的16S rDNA序列相似度达99.50%。The strains with antagonistic activity against Verticillium dahliae Kleb. were screened from the soil. Of the 68 strains obtained, a strain named DS45-2 with a relatively higher antagonistic activity. The strain DS45-2 was further purified and characterized by morphological and culture observation, physiological and biochemical experiments and 16S rDNA sequence analysis. Based on the results, the strain was initially identified as a Bacillus amytoliquefaciens. The similarity of the 16S rDNA sequences between strain DS45 2 and the type strain NBRC 15535 of Bacillus amyloliquefaciens was close to 99.50%.

关 键 词：大丽轮枝菌 拮抗细菌 分离 鉴定 解淀粉芽孢杆菌 

分 类 号：S435.621.24[农业科学—农业昆虫与害虫防治]