藏獒群体遗传结构及遗传分化研究  

Genetic Structure and Genetic Differentiation of Tibetan Mastiff Populations

作　　者：兰小平[1] 郭宪[2] 陈永昌[3] 杨俊年[4] 鄢珣[5] 崔泰保[5] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741000 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [3]中国科学院昆明动物研究所,云南昆明650223 [4]重庆三峡学院生物系,重庆404000 [5]甘肃农业大学,甘肃兰州730070

出　　处：《河南农业科学》2010年第1期110-112,共3页Journal of Henan Agricultural Sciences

基　　金：甘肃省科技厅基金资助项目(GKC-97-27-5);天水师范学院青年教师基金资助项目

摘　　要：采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对河曲藏獒和青海藏獒2个群体86只犬的9个结构基因座(Tf、Po、Es-1、Es-2、Sα2、Hb、Alb、Pr、Amy)的遗传变异情况进行了检测,探讨了2个藏獒群体的遗传结构和遗传分化。结果表明,河曲藏獒群体内遗传变异较青海藏獒丰富,而2个藏獒群体间的遗传分化程度很低(GST=0.018 7);较大的基因流(Nm=14.543 7)是2个藏獒不同地理群体间遗传分化水平低的主要原因。A total of 86 blood samples were taken from two populations of Hequ Tibetan Mastiff and Qinhai Tibetan Mastiff. The genetic structure and genetic differentiation of nine structural loci of Tf, Po, Es-1, ES-2, Sa2, Hb, Alb, Pr and Amy between these two populations were examined by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. The results showed that the genetic diversities within the population of Hequ Tibetan Mastiff were richer than that of Qinhai Tibetan Mastiff, and the genetic differentiation is very limited between Tibetan Mastiff populations （GST =0. 018 7）. High gene flow （Nm = 14. 543 7） was the main reason leading to the very low genetic differentiation level of different populations of the Tibetan Mastiff.

关 键 词：藏獒 遗传结构 基因流 遗传分化 

分 类 号：S829.2[农业科学—畜牧学]