牛生长激素BGH基因的克隆及其真核表达载体pEGFP-N1-BGH的构建  

Cloning of BGH Gene and Construction of a Eukaryotic Expression Vector pEGFP-N1-BGH

作　　者：韦光辉[1] 徐廷生[1] 雷雪芹[1] 索剑飞[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《河南农业科学》2010年第1期113-115,共3页Journal of Henan Agricultural Sciences

基　　金：河南省基础与前沿技术研究计划项目(072300430010)

摘　　要：采用逆转录聚合酶链式反应(RT-PCR)技术,从11月龄的牛脑垂体组织中扩增出BGH基因片段,通过DNA重组技术将该基因片段重组于pEGFP-N1真核表达载体上,构建pEGFP-N1-BGH重组质粒。通过PCR扩增、酶切电泳分析和测序的方法对重组DNA进行鉴定,结果表明,成功构建了pEGFP-N1-BGH真核表达载体。The bovine growth hormone （BGH） gene was amplified from the tissue of calf brain pituitary by RT - PCR and cloned into the eukaryotic expression vector pEGFP - N1. The recombinants were further identified by PCR amplification, enzyme digestion and DNA sequencing. The results showed that the eukaryotic expression vector pEGFP- N1 was successfully constructed.

关 键 词：牛生长激素基因 克隆 真核表达载体pEGFP—N1 载体构建 

分 类 号：S823[农业科学—畜牧学] Q782[农业科学—畜牧兽医]