黄芩苷调节DN大鼠肾脏局部生物活性物质的实验研究  

Experimental Study of Baicalin on Regulation of Local Bioactive Substances in Kidney of DN Rat

作　　者：苏宁[1] 李丰[1] 赵平[1] 周乐全[1] 邝枣园[1] 杜标炎[1] 罗荣敬[1] 

机构地区：[1]广州中医药大学基础医学院,广东广州510006

出　　处：《世界中西医结合杂志》2010年第1期22-24,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：广东省中医药管理局课题(No.103031)

摘　　要：目的研究黄芩苷对糖尿病肾病(DN)大鼠肾脏局部生物活性物质的调节作用。方法采用链尿佐菌素单次腹腔注射法制作DN模型大鼠,随机分为模型组、黄芩苷低剂量组及高剂量组,另设正常对照组。黄芩苷低、高剂量组大鼠分别腹腔注射黄芩苷水溶液40 mg.kg-1.d-1、60mg.kg-1.d-1。治疗6 w后,取各组大鼠血液及肾脏,检测血AngⅡ、肾脏TGF-β1及PKC活性。结果黄芩苷低、高剂量组血AngⅡ降低,与模型组比较有显著性差异(P<0.05)。黄芩苷低、高剂量组肾小管TGF-β1表达与模型组比较明显减少,肾小管上皮细胞膜PKC表达与模型组比较减少,其中以黄芩苷低剂量组更接近正常对照组的表现。结论黄芩苷可降低血AngⅡ,对改善肾脏血流动力学有一定帮助。黄芩苷可降低肾组织内增高的TGF-β1、PKC,从而调节细胞外基质(ECM)的沉积和降解。Objective To study the regulation of baicalin in local bioactive substances in kidney of rat with diabetic nephropathy（ DN）. Methods DN rat model was established by single intraperitoneal injection of streptozotocin. DN model rats were randomly divided into model group,baicalin low - dose group and baicalin high - dose group. Normal control group was set up additionally. Rats in baicalin low - dose group and high - dose group were injected with intraperitoneally baicalin solution 40mg/kg/day and 60mg/kg/day respectively. Six weeks later, blood and kidneys were collected from each group. Blood Ang II , kidney TGF - β1 and PKC activity were determined. Results Aug II in blood decreased in low - dose and high - dose groups,indicating significant difference compared with model group（ P 〈 0. 05 ）. Tubule TGF- β1 expression in low- dose and high- dose groups decreased apparently compared with model group and tubule epithelial cell membrane PKC expression decreased compared with model group, of which,the expression in low - dose group was much closer to that in normal group. Conclusion Baicaliu reduces Ang II in blood and improves renal hemodynamics to a certain extent. Baicalin decreases the increased expression of TGF - β1 and PKC in renal tissue so that the deposition and degradation of extracellular matrixc（ECM） can be adjusted.

关 键 词：黄芩苷 糖尿病肾病 TGF-β1 PKC AngⅡ 

分 类 号：R692[医药卫生—泌尿科学] R282.71[医药卫生—外科学]