中药牛蒡对蛋白非酶糖化、肿瘤坏死因子α的影响及防治糖尿病肾病的作用  

The influevent of arctium Lappa Lon non-enzymatic glycation of protein,tumor necrosis factor-α and it's preventive effect of diabetic nephropathy

作　　者：李汶娟[1] 王桂霞[1] 王秀军[1] 刘长山[1] 

机构地区：[1]潍坊医学院附属潍坊市人民医院内分泌科,山东潍坊261041

出　　处：《中国当代医药》2010年第3期9-10,19,共3页China Modern Medicine

摘　　要：目的:观察中药对糖尿病大鼠蛋白非酶糖化、肿瘤坏死因子α(TNF-α)及尿清蛋白的影响,探讨其对糖尿病肾病(DN)的防治作用及可能的作用机制。方法:雄性Wistar大鼠30只,随机平均分为3组,正常对照组、糖尿病对照组、糖尿病牛蒡[10ml/(kg·d)]治疗组,腹腔注射链脲佐菌素(60mg/kg)诱发糖尿病。于4、8、12、16周测尿清蛋白,16周后,取血测血糖、TNF-α,处死大鼠,分离肾脏,测定组织非酶糖化。结果:糖尿病组大鼠尿清蛋白有上升趋势,牛蒡治疗组尿清蛋白基本维持不变,牛蒡治疗组蛋白非酶糖化受抑制,TNF-α表达下降。结论:牛蒡可以降低尿蛋白,减轻肾脏损害,其防治DN的机制可能与抑制蛋白质非酶糖化,减少细胞因子的产生有关。Objective： To observe the effects on protein non-enzymatic glycation in diabetic rats, tumor necrosis factor-α （TNF-α） and urine albumin, and to explore the development of diabetic nephropathy （DN） of the preventive effect and possible mechanism of action. Methods： Male Wistar 30 rats were randomly divided into three groups, normal control group, diabetes control group, group diabetic rats treated with arctium Lappa L [（10 ml/kg·d）; intraperitoneal injection of streptozotocin （60 mg/kg）induced diabetes. Urine protein measured at 4,8,12,16 weeks, 16 weeks later, blood glucose measurement, TNF-α, rats were killed, separation kidney, measured organizations, non-enzymatic glycation. Results： The diabetic rats urinary albumin upward trend, arctium Lappa L treatment of urinary albumin essentially unchanged, arctium Lappa L treatment group protein non-enzymatic glycosylation was inhibited, TNF-α expression decreased. Conclusion： arctium Lappa L can reduce urinary protein, to reduce kidney damage, its prevention and control mechanism of DN may inhibit non-enzymatic glycation of protein, reducing the production of cytokines.

关 键 词：非酶糖化 肿瘤坏死因子α  糖尿病肾病 牛蒡 

分 类 号：R587.2[医药卫生—内分泌]