白附片对正常和肾阳虚大鼠血液生化指标的影响  

Influence of White Prepared Lateral Root of Aconite on Blood Biochemical Indicators in Normal and Kidney Yang Deficient Rats

作　　者：谭勇[1] 吕诚[1] 赵宏艳[2] 丁晓蓉[1] 李小冰[1] 鞠大宏[2] 吕爱平[1] 

机构地区：[1]中国中医科学院中医临床基础医学研究所,北京100700 [2]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国中医药信息杂志》2010年第1期31-33,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：第43批博士后科学基金资助(20080430533);国家"十一五"科技支撑计划重点课题(2006BAI08B01-01);北京市教育委员会科技研究面上项目(KM200910025012)

摘　　要：目的探索白附片对正常和肾阳虚大鼠血液生化指标影响的差异。方法80只SD雄性大鼠随机平均分为肾阳虚模型组和正常组;采用肌注氢化可的松琥珀酸钠的方法造模。造模结束后,将2组大鼠各自随机分为低剂量、小剂量、中剂量和大剂量给药组和空白对照组,每组8只;对各给药组大鼠连续14d灌胃给予不同浓度的白附片溶液。末次给药24h后取血进行放免和生化检测。结果给药前模型组环磷酸腺苷(cAMP)/环磷酸鸟苷(cGMP)和促肾上腺皮质激素(ACTH)低于正常组,随着给药剂量的增加,两者有升高的趋势;给药前模型组血糖(GLU)低于正常组,随着给药剂量的增加,模型组GLU有升高的趋势,而正常组有下降的趋势;中剂量给药时模型组总胆固醇(CHO)显著高于正常组;随着给药剂量的增加,2组丙氨酸氨基转移酶(ALT)均有升高的趋势,且正常组ALT升高的幅度更大;给药前模型组尿素氮(BUN)高于正常组,低、小剂量给药有降低模型组BUN的趋势。结论白附片对正常大鼠和肾阳虚模型大鼠血液生化指标的影响不同,对正常大鼠的毒性效应更明显。Objective To explore the differences of blood biochemical indicators between normal and kidney Yang deficient rats administered orally different doses of white prepared lateral root of aconite. Method Eighty Sprague-Dawley （8D） male rats were divided randomly into normal group and kidney Yang deficient model group, forty rats in each group. After producing kidney Yang deficient model by injecting hydrocortisoni natrii succinas in intramuscular, normal and model rats were respectively and randomly divided into little, low, medium, great dose group and blank group, eight rats in each group. Those rats were administered orally with different concentrations of white prepared lateral root of aconite every day for two weeks. Twenty-four hours after the last oral administration, the blood were obtained and used for testing adrenocorticotrophic hormone （ACTH）, cyclic adenosine monophosphate （cAMP）, cyclic guanosine monophosphate （cGMP）, glucose （GLU）, cholesterol （CHO）, alanine aminotransferase （ALT）, urea nitrogen （BUN）. Results Before taking medicine, model rats＇ cAMP/cGMP and ACTH were lower than normal rats＇, and they showed a trend of rise with adding dose. Before taking medicine, model rats＇ GLU was lower than normal rats＇. With adding dose, model rats＇ GLU increased, however, normal rats＇ GLU reduced. When taken medium dose white prepared lateral root of aconite, model rats＇ CHO was higher than normal rats＇. Taking medicine made both groups ALT rise, but normal rats＇ went up more quickly than model rats＇. Model rats＇ BUN was higher than normal rats＇, and taking low dose or little dose medicine could lead to incline of reduction. Conclusion White prepared lateral root of aconite caused different blood biochemical effects on normal and kidney Yang deficient rats, and more remarkable toxicity effect on normal rats.

关 键 词：白附片 肾阳虚模型 血液生化指标 大鼠 

分 类 号：R285.5[医药卫生—中药学]