地锦草不同萃取部位体外抗真菌作用研究  

Study on Antifungal Susceptibility of Different Extract Parts of Euphorbia humifusa

作　　者：安惠霞[1] 斯拉甫.艾白 古力娜.达吾提 李治建 

机构地区：[1]石河子大学药学院,新疆石河子832000 [2]新疆维吾尔自治区维吾尔医药研究所,新疆乌鲁木齐830049 [3]新疆维吾尔自治区维吾尔医医院,新疆乌鲁木齐830049

出　　处：《中国中医药信息杂志》2010年第1期40-42,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划(2007BAI30B03);国家自然科学基金(30760308)

摘　　要：目的对地锦草不同萃取部位进行体外抗真菌实验,测定其最小抑菌浓度值(MIC)。方法采用溶剂萃取法分离地锦草乙醇提取液,得到水、石油醚、氯仿、正丁醇等4个萃取部位;根据美国临床实验室标准化委员会(NCCLS)推荐的《产孢丝状真菌的液基稀释法抗真菌药物敏感性试验方案》(M38-A),测定地锦草各萃取部位对23株临床常见皮肤癣菌的MIC。结果由于地锦草萃取分离后的石油醚和氯仿部位在药敏试验中的溶解度甚小,影响结果的科学观察,本研究主要针对正丁醇和水部位的体外药敏进行了检测,正丁醇和水部位对真菌有明显的抑制作用,并且正丁醇部位的作用优于水部位。结论地锦草萃取部位具有体外抗真菌作用。Objective： To examine the antifungal effect of different extraction parts of Euphorbia humifusa in vitro, and measure the minimum inhibition concentrations （MICs）. Methods Separated the Dijincao extract and got four parts by solvent extraction. Applied the standard which was approved by the National Committee for Clinical Laboratory Standards （NCCLS）： Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous, Fungi （M38-A）. MICs of different parts of Dijincao extract against 23 strains of common clinical dermatophytes were investigated. Results Chloroform extract and petroleum ether extract could not get clear and defipite MIC because their solubility disturbed the determination of experiment＇s results. However, water and n-butanol fraction could get definite and clear results. Both of these two parts could clearly inhibit fungal growth. Conclusion Euphorbia humifusa extract parts have antifungnal activity against dermatophytes in vitro.

关 键 词：地锦草 M38-A方案 体外试验 抗真菌作用 最低抑菌浓度 

分 类 号：R284.2[医药卫生—中药学]