慢性心力衰竭肾阳虚证患者代谢组学研究  

Research on metabolomics in chronic heart failure with kidney-yang deficiency

作　　者：郑海生[1] 蒋健[1] 贾伟[2] 贺敏[1] 蒋梅先[1] 阮小芬[1] 苏明明[2] 吴建兵[3] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021 [2]上海交通大学药学院,上海202402 [3]上海中医药大学药学院,上海201203

出　　处：《中华中医药杂志》2010年第2期198-201,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30572286);上海市教委重点学科中药临床药理学(No.J50303);上海市申康医院发展中心临床药理学科研共享平台(No.SHDC12007202);上海市浦东新区科技局新药临床试验研究平台(No.PDPT2005-03)~~

摘　　要：目的:研究慢性心力衰竭肾阳虚证患者的代谢模式,探讨代谢组学在中医证候学研究中的应用。方法:收集慢性心力衰竭患者54例(其中肾阳虚证27例、非肾阳虚证27例)及正常人24例的空腹晨尿尿样,通过气相色谱/质谱(GC/MS)联用及模式识别为主的代谢组学方法进行研究。结果:主成分分析和正交最小二乘法分析中慢性心力衰竭肾阳虚证组、非肾阳虚证组和正常组的代谢成分积分在空间分布上明显分开,且样本均投影到2SD(标准差)的区域内,表示其值可信,说明所建模型能准确区分3者。慢性心力衰竭患者与正常人比较还发现了柠檬酸、丙氨酸、3-甲基戊烯二酸、丙胺、组胺5种差异性代谢物质。结论:慢性心力衰竭肾阳虚证患者、非肾阳虚证患者和正常人具有不同的代谢模式,以GC/MS为技术特点的代谢组学研究方法能将3者区分。代谢组学是研究中医学证本质的有效方法。Objective： To investigate metabolic patterns of chronic heart failure with kidney-yang deficiency and discuss the application of metabolomics in study of TCM syndrome. Methods： The urine samples of 24 healthy volunteers and 57 patients with chronic heart failure were studied （kidney yang deficiency 27 and non-kidney yang deficiency 27） through multiples bioinformatics processing technology, including metabolomics method, chromatography-mass spectrometry technique, as well as complex chemical, biology pattern recognition and data mining. Results： Principal components analysis and orthogonal partial least square discriminant analysis found that singulorum sample metabolism principal constituent score spot spatial distribution indicated kidney-yang deficiency, no kidney-yang deficiency and normal control had significant difference. Singulorum sample metabolism principal constituent score less than 2 times the standard deviation, the results showed that the model can distinguish kidney-yang deficiency and non-kidney-yang deficiency.Chronic heart failure group compared with the control group, displayed significant changes in citric acid, alanine, hmgcoa, propylamine, histamine. Conclusion： Metabolomic pattern of kidney yang deficiency and non-kidney yang deficiency were different. Kidney yang deficiency and non-kidney yang deficiency can be distinguished by multiples bioinformatics processing technology. Therefore, metabolomics can provide a new space for the development of TCM syndrome.

关 键 词：慢性心力衰竭 肾阳虚证 代谢组学 

分 类 号：R259[医药卫生—中西医结合]