高效液相色谱法测定不同产地板蓝根中2，4（^1H，^3H）喹唑二酮含量  

Content determination of 2,4（^1H,^3H）-quinazolinedione in Radix Isatidis from different regions by HPLC

作　　者：安益强[1,2] 贾晓斌[1,2] 陈妍桦[3] 高成林[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室国家中医药管理局名医名方适宜剂型重点研究室,南京210028 [2]江苏大学药学院,镇江212013 [3]南京中医药大学药学院,南京210046

出　　处：《中华中医药杂志》2010年第2期275-277,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省中医药领军人才资助项目(No.2006);苏州市科技计划(No.SSY0606)~~

摘　　要：目的:建立HPLC法测定板蓝根药材中2,4(1H,3H)喹唑二酮含量的方法,为板蓝根药材的质量控制提供依据。方法:采用高效液相色谱方法,色谱柱为ZORBAXSB-C18(4.6mm×250mm,5μm);流动相为乙腈-(0.1%磷酸+0.03%三乙胺)水溶液=12∶88;流速为0.7mL/min;检测波长为224nm,柱温为30℃。结果:2,4(1H,3H)喹唑二酮线性范围为0.0106-0.1696μg(r=0.9995),平均加样回收率为98.53%,RSD为1.13%。结论:建立了高效液相测定板蓝根药材2,4(1H,3H)喹唑二酮含量的方法,该方法准确、可靠,可用于板蓝根药材的质量控制;不同产地板蓝根中2,4(1H,3H)喹唑二酮的含量差异较大。Objective： To establish an HPLC method for determination of the content of 2,4（^1H,^3H）-quinazolinedione in Radix Isatidis and provide valuable data for quality control of Radix Isatidis.Methods：The samples were separated on an ZORBAX SB-C18.4.6mm×250mm, 5μm, column with the mobile phase of acetonitrile： （0.1% phosphoric acid ＋ 0.03% triethylamine）, aqueous solution=12：88.Flow rate was 0.7mL/min.The detection wavelength was set at 224nm. Column temperature was 30.Results：The linear range of 2,4 （^1H,3H）quinazolinedione was 0.0106μg, 0.1696μg r=-0.9995 the average recovery was 98.53%, RSD was 1.13%（n=9）.Conclusion：The method for quantitation of 2,4（^1H,^3H）quinazolinedione in Radix Isatidis was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis. The difference that the content of 2,4（^1H,^3H）-quinazolinedione in Radix Isatidis from different origins is obvious.

关 键 词：板蓝根 2 4(^1H ^3H)喹唑二酮 HPLC 制备 含量测定 

分 类 号：R284.1[医药卫生—中药学]