糖足合剂对瘀血阻滞证糖尿病足大鼠的治疗作用及机制  

Mechanism and effect of Tangzu Heji on diabetic foot rat with blood stasis syndrome

作　　者：陶茂灿[1] 黄平[1] 许经论 高娟娟[3] 曹毅[1] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310006 [2]浙江金华市第五人民医院,金华321000 [3]河北省邢台市人民医院,邢台054031

出　　处：《中华中医药杂志》2010年第2期291-295,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药管理局资助项目(No.2008CA011)~~

摘　　要：目的:探讨中药糖足合剂对瘀血阻滞证糖尿病足大鼠的治疗作用及机制。方法:采用注射链脲佐菌素加降低环境温度方法形成瘀血阻滞证糖尿病足大鼠模型。随机将60只SD雄性大鼠分为正常对照组,正常对照降温组,糖尿病足组(模型组),糖足合剂高、中、低剂量组共6组,造模成功后,前3组给予生理盐水,后3组分别给予糖足合剂60g/kg、30g/kg、15g/kg体质量,治疗2周,观察大鼠体质量、饮水量、血糖、血液流变学、肢端坏疽症状、舌下脉络和耳廓血管及血清血管内皮生长因子的变化。结果:经糖足合剂治疗2周后,与模型组相比,体质量明显增加、饮水量明显减少、低剂量治疗组红细胞聚集指数明显下降(P<0.05),空腹血糖、全血粘度高切、中切、低切和高、中剂量治疗组红细胞聚集指数、肢端坏疽症状评分明显降低(P<0.01),血清血管内皮生长因子浓度明显升高(P<0.01)。与治疗前相比,饮水量明显减少(P<0.05),空腹血糖、肢端坏疽症状评分明显降低(P<0.01),高剂量治疗组体质量明显增加(P<0.05)。结论:糖足合剂对瘀血阻滞证糖尿病足具有治疗作用,可能通过降糖,阻滞糖尿病足的发展进程,降低血液流变学参数,改善血液循环,提高血清血管内皮生长因子,促进糖尿病足闭塞血管周围侧支增生,增加血供,促进溃疡愈合而起作用。Objective： To observe the effect on Tangzu Heji on diabetic foot rat with blood stasis syndrome and to investigate the mechanism in it. Methods： Combination of streptozocin injection and reduction of ambient temperature was used to duplicate diabetic foot rat with blood stasis syndrome. 60 Male SD rats were randomly divided into 6 groups as normal control group, the low temperature control group, diabetic foot group （model group）, three groups of Tangzu Heji treatment with high, medium and low dose respectively. In the first three groups the rats were given normal saline, and in the latter three treatment groups the model rats were given Tangzu Heji as 60gkg, 30g/kg, 15g/kg weight, respectively. After two weeks of treatment, the changes of the weight, water intake, blood glucose, blood rheology, acral gangrene symptoms, Choroid blood vessels of the sublingual and ear, serum vascular endothelial growth factor changes were observed. Results： After two weeks of treatment with Tangzu Heji, compared with the model group, a marked increase in body weight, decrease of water intake were happened to the rats in treatment groups. Erythrocyte aggregation index in the low-dose treatment group decreased significantly （P〈0.05）. Fasting blood glucose, high, middle and low shear whole blood viscosity and erythrocyte aggregation index and acral gangrene symptom score in the high-dose and middle-dose treatment groups decreased significantly （P〈0.01）, serum vascular endothelial growth factor concentration significantly increased （P〈0.01）. Compared with pre-treatment, water intake decreased significantly （P〈0.05）, fasting blood glucose and acral gangrene symptom score decreased significantly （P〈0.01）, body weight of the rats in high-dose treatment group significantly increased （P〈0.05）. Conclusion： Tangzu Heji had treatment effect on diabetic foot rat with blood stasis syndrome. Tangzu Heji may block the development process of diabetic foot through decreasing the blood glucose, and pro

关 键 词：糖尿病足 中医药治疗 瘀血阻滞证 血管内皮生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]