三联放大技术与荧光定量PCR检测乙型肝炎病毒的应用比较  

Comparing PCR coupled with blot-hybridization and enzyme linking to FQ-PCR to detect HBV

作　　者：唐九歌[1] 丁剑冰[1] 郭劲 乔艳辉 袁晓华 何智 郭伟鹏 

机构地区：[1]新疆医科大学基础医学院,新疆乌鲁木齐830054 [2]乌鲁木齐血液中心,新疆乌鲁木齐830000

出　　处：《中国病原生物学杂志》2010年第1期16-19,共4页Journal of Pathogen Biology

基　　金：新疆高技术研发项目(No.20061110;PT0805)

摘　　要：目的通过比较聚合酶链反应偶联印迹杂交和酶联放大技术(即三联放大技术)与荧光定量PCR(FQ-PCR)两种方法检测HBV DNA结果,探讨三联放大技术用于血液HBV筛查的优越性。方法医院肝病门诊患者血清195份,同时进行三联放大技术检测和FQ-PCR检测,比较检测结果。结果195份血清,经三联放大技术检出HBVDNA 125份,检出率64.10%;FQ-PCR检出HBV DNA 103份,检出率52.82%。两种方法的HBV DNA检出率差异有统计学意义(P<0.01)。结论与FQ-PCR比较,三联放大技术能显著提高HBV DNA的检出率,可用于血液HBV筛查。Objective To compare the results of HBV DNA detection with two techniques： polymerase chain reaction coupled with blot-hybridization and enzyme linking （the Triple Amplification Technique） and fluorescence quantitative PCR （FQ-PCR）. Discussion The Triple Amplification Technique detected HBV in screened blood donors. Methods The Triple Amplification Technique and FQ-PCR detected 195 serum samples from hepatitis patient in clinic of the hospital, then compared to testing result. Results The Triple Amplification Technique detected HBV DNA in 125 of 195 serum samples for a positive detection rate of 64.10%. FQ-PCR detected HBV DNA in 103 of 195 serum samples for a positive detection rate of 52.82%. There was a statistically significant difference （P〈0.01） between the two methods. Conclusion Compared to FQ-PCR, the Triple Amplification Technique can increase the detection rate of HBV DNA and thus should be used to detect HBV when screening blood donors.

关 键 词：三联放大技术 荧光定量PCR 肝炎病毒 乙型 血液 检测 

分 类 号：R372.21[医药卫生—病原生物学]