木香、黄精提取液抑制R质粒接合传递的体外实验研究  

In vitro study of the inhibition of R plasmid transfer by conjugation as a result of taking extracts of Radix aucklandiae and Polygonatum cyrtonema

作　　者：李玉虎[1] 龚甜[1] 侯庆萍[1] 程国强[1] 缪珠雷[1] 蒋美娟[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国病原生物学杂志》2010年第1期26-28,73,I0005,共5页Journal of Pathogen Biology

基　　金：上海市教委重点学科;中西医结合基础(No.J50301);上海市教委高校高水平特色发展项目[沪教委财(2005)81号]

摘　　要：目的体外观察中药木香、黄精提取液抑制痢疾杆菌氯霉素耐药株(S.flexneRiD15 R-plasmid CmR)和大肠埃希菌氯霉素敏感株(E.coli1485 RifR)间的R质粒接合传递作用。方法木香、黄精提取液分别与S.flexneRiD15 R-plasmid CmR和E.coli1485 RifR共同培养24 h和48 h后接种于麦康凯琼脂平板,37℃培养24 h,计算木香、黄精及不加药对照组的R质粒转移率和R质粒转移抑制率。结果24 h R质粒转移率木香组为(4.60±0.14)%,黄精组为(4.15±0.78)%,未加药对照组为(47.3±0.53)%,差异有统计学意义(P<0.01);48 h R质粒转移率木香组为(3.65±0.07)%,黄精组为(2.8±0.71)%,未加药对照组为(68.90±1.01)%,差异有统计学意义(P<0.01)。24 h的R质粒转移抑制率木香组为(90.30±8.33)%,黄精组为(91.22±7.76)%;48 h的R质粒转移抑制率木香组为(94.70±9.68)%,黄精组为(95.90±10.23)%。结论木香、黄精均具有体外抑制S.flexneRiD15 R-plasmid CmR和E.coli1485 RifR间R质粒接合传递的作用。Objective To observe the effective inhibition of R plasmid transfer by conjugation from S. flexneri D15 R- plasmid Cm^R to E. coli 1485 Rif^R in vitro as a result of taking extracts of Chinese medicines. Methods Extracts of Radix aucklandiae and Polygonatum cyrtonema were cocuhured with S. flexneri D15 R-plasmid Cm^R and E. coli 1485 Rif^R for twenty-four hours and forty-eight hours and then seeded on MacConkey agar for 24 hours at 37 ℃. Then, the transfer rate and the inhibition rate of R plasmid of the two medicines were calculated in comparison to the control group. Results The transfer rate of R plasmid（%）in 24 hours was 4.60±0. 14 （R. aucklandiae） and 4.15±0.78（P. cyrtonema）, and there was a very significant statistical difference between the transfer rate of R plasmid in the groups taking the extracts and the control group not taking the extracts of 47.3±0.53 （P〈0.01）. The transfer rate of R plasmid（ % ） in 48 hours was 3.65±0.07 （R. aucklandiae） and 2.8±0.71 （P. cyrtonema）, and there was a very significant statistical difference between the transfer rate of R plasmid in the groups taking the extracts and the control group not taking the extracts of 68.9±1.01 （P〈0.01）. The inhibition rate（%） in 24 hours was 90.30±8.33 （R. aucklandiae） and 91.22± 7.76 （P. cyrtonerna）; the inhibition rate（%） in 48 hours was 94.70±9.68 （R. aucklandiae） and 95.90±10.23 （P. cyrtonema）. Conclusion Extracts of R. aucklandiae and P. cyrtonema can effectively inhibit conjugative R plasmid transfer from S. flexneri D15 R-plasmid Cm^R to E. coli 1485 Rif^R in vitro.

关 键 词：R质粒 抑制接合传递 S.flexneRi D15 R-plasmid Cm^R E.COLI 1485 Rif^R 

分 类 号：Q5[生物学—生物化学]