美托洛尔对充血性心力衰竭大鼠血浆醛固酮浓度、心肌纤维化和心室重构的影响  

Effect of Metoprolol on Change of Plasma Aldosterone,Myocardium Fibrosis and Myocardial in Rats with Remodeling Congestive Heart Failure

作　　者：蔡辉[1] 李筝[1] 焦东东[1] 郭郡浩[1] 郭寒[1] 

机构地区：[1]南京军区南京总医院中西医结合科,南京210002

出　　处：《微循环学杂志》2010年第1期13-15,F0003,共4页Chinese Journal of Microcirculation

基　　金：南京军区南京总医院科研基金资助项目(NO:2008042)

摘　　要：目的:探讨美托洛尔对充血性心力衰竭(CHF)大鼠血浆醛固酮(ALD)浓度的影响及防治心肌纤维化及心室重构的作用。方法:雄性SD大鼠30只,随机选择20只皮下注射异丙基肾上腺素二次(170mg/kg/次),6周后左室射血分数≤45%确定造模成功;将模型大鼠随机分为模型组(n=10)和美托洛尔治疗组(n=10,8mg/kg/天,共治疗12周);另10只大鼠作为正常对照组。18周后进行心功能指标、血浆ALD浓度检测,并取心肌观察组织形态学及胶原纤维染色。结果:(1)与对照组相比,模型组心功能指标明显恶化,血浆ALD显著升高(P<0.01),心肌纤维化和心室重构明显;(2)与模型组比较,美托洛尔组心功能明显好转,血浆ALD明显下降(P<0.01),心肌纤维化和心室重构明显改善。结论:美托洛尔能通过降低CHF大鼠血浆ALD浓度而改善心功能,逆转心室重塑。Objective:To investigate the effect of metoprolol on plasma aldosterone,myocardium fibrosis and reverse ventricular remodeling in rats with corgestive heart failure.Method:Male SD rats were randomly allocated to the Isopreternol(ISO)-induced chronic heart failure group(n=20)receiving two subcutaneous injections of 170mg ISO per kilogram of body and the healthy normal group(n=10)receiving two subcutaneous injections of 0.25ml normal saline.At 6th week,the LVEF was measured by echocardiogram,the remaining rats with LVEF≤45% were randomly divided into two groups:control group(n=10),metoprolol group 8 mg/kg/d(n=10).At 18th week,the HR,LVEF,LVEDP,LVSP were assessed.Aldosterone(ALD)in plasma were examined by radio-immunity method,and the mass ratio of heart ventricle and left ventricle to body was measured.Results:(1)Compared with the normal group,the HR,LVEDP and ALD in plasma were significantly increased(P<0.01).But LVSP and ±dp/dtmax were significantly decreased in the control group.(2)Compared with the control group,the HR,LVEDP,LVWI and ALD in plasma were decreased distinctly.The LVSP and ±dp/dtmax were increased distinctly in the metoprolol group(P<0.01).Conclusion:Metoprolol can inhibit ALD in plasma,improve heart function and reverse ventricular remodeling with congestive heart failure.

关 键 词：充血性心力衰竭 血浆醛固酮 心肌纤维化 美托洛尔 心室重构 肾素一血管紧张素系统 心肌间质纤维化 心血管疾病 

分 类 号：R541.61[医药卫生—心血管疾病] R972.1[医药卫生—内科学]