健康人群肿瘤坏死因子-α基因多态性的分析  

Analysis on polymorphisms of TNF-α gene in healthy population

作　　者：向瑜[1] 杨致邦[1] 李永国[2] 韩飞[1] 苏善平[1] 郭丽媛[1] 

机构地区：[1]重庆医科大学基础医学院病原生物学教研室神经科学研究中心基础医学实验教学中心病原生物学与免疫学实验室 [2]重庆医科大学基础医学院法医学教研室,重庆400016

出　　处：《生物学杂志》2010年第1期24-26,共3页Journal of Biology

摘　　要：了解汉族健康人群中TNF-A基因多态性的分布,研究TNF-α表达与相关疾病之间的联系。采用PCR-限制性长度片段多态分析法检测140名重庆地区汉族健康人群的TNF-A-308,TNF-A-857位点基因多态性,计算其基因型和等位基因频率,结果显示TNF-A-308 G/G、G/A、A/A基因型的频率分别为89%、11%、1%,其等位基因的发生频率以G等位基因最常见(93%),其次为A等位基因(7%)。TNF-A-857 C/C、C/T、T/T基因型的频率分别为68%、36%、8%,其等位基因发生频率以C等位基因最常见(81%),其次为T等位基因(19%)。由结果可以得出重庆地区汉族健康人群TNF-A-308位点存在G/A多态性,TNF-A-857位点存在C/T多态性。To observe the polymorphisms of TNF-α genes in Han healthy people and establish the base on relationship between the expressions of TNF-α and certain disease, gene polymorphisms of TNF-A-308 and TNF-A-857 were genotyped by the PCR-RFLP method. The genotypes and allele frequencies were calculated and analyzed. In Han population, the frequencies of three TNF-A-308 genotype of G/G, G/A and A/A were 89% , 11% and 1% , respectively, and the frequencies of allele G （93%） were most commonly seen followed by the frequencies of allele A （7%）. The frequencies of TNF-A-857 genotype of C/C, C/T and T/T were 68% , 36% and 8% , respectively, and the frequencies of allele C （81%） was most commonly seen followed by the frequencies of allele T （ 19% ）. It indicated that among the Han population in Chongqing, the TNF-A-308 site exists G/A polymorphism. In addition, the TNF-A-857 site exists C/T polymorphism.

关 键 词：基因多态性 肿瘤坏死因子-Α 

分 类 号：R378[医药卫生—病原生物学]