续断药材指纹图谱的研究  

Research on Fingerprint of Radix Dipsaci by HPLC

作　　者：曹越[1] 彭维[2] 苏薇薇[2] 罗颂平[1] 

机构地区：[1]广州中医药大学第一临床医学院,广州510405 [2]中山大学生命科学学院,广州510275

出　　处：《中药新药与临床药理》2010年第1期50-54,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(30772819)

摘　　要：目的建立续断药材的高效液相色谱(HPLC)指纹图谱,为科学评价及有效控制续断药材质量提供可靠方法。方法采用高效液相色谱法,选用Agilent HC-C18色谱柱,流动相为乙腈(A)-(0.05mol/L)KH2PO4-H3PO4缓冲溶液pH=3.0(B),线性洗脱梯度,检测波长为220nm;流速为1.0mL/min。结果分析了10批不同时间不同产地的道地续断药材,建立了续断药材的HPLC指纹图谱,确立了17个共有峰,并且利用"中药色谱指纹图谱相似度评价软件"对不同样品之间的相似度进行计算。结论该方法准确可靠,重复性好,为续断药材的质量控制提供科学准确的依据。Objective To establish chromatographic fingerprint of water and ethanol soluble component of Radix Dipsaci by using HPLC for identifying and evaluating its quality. Methods HPLC method was set up with Agilent HC - C18 column. The mobile phase was comprised of acetonitrile（A） and 0.05mol/L KH2PO4 - H3PO4 buffer solution pH = 3.0（B） in gradient elution mode. UV detection wavelength was at 220 nm, with a flow rate of 1.0 mL/min. Results Ten batches of Radix Dipsaci samples from different habitats and harvested in different times were analyzed. HPLC fingerprint of medicinal material of Radix Dipsaei was established preliminarily. Seventeen characteristic peaks were confirmed and the fingerprints of different samples were compared by similarity evaluation software. Conclusion This method shows high precision and good repeatability, so it can be used to assess the quality of Radix Dipsaci.

关 键 词：续断 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]