大孔吸附树脂纯化侧柏叶总黄酮的工艺研究  

Purification of the Total Flavonoids from Cacumen Platycladi with Macroporous Adsorption Resin

作　　者：黄月纯[1] 黄樱华[1] 刘翠玲[2] 陈慕媛[2] 魏刚[2] 刘东辉[2] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学,广州510405

出　　处：《中药新药与临床药理》2010年第1期75-80,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家"十一五"科技支撑计划项目(2008BAI53B074)

摘　　要：目的研究AB-8型大孔树脂精制侧柏叶总黄酮的工艺条件。方法以总黄酮、槲皮苷含量结合指纹图谱主要特征峰面积保留率为考察指标;采用可见分光光度法测定总黄酮含量,HPLC法测定槲皮苷含量,HPLC法分析指纹图谱主要特征峰面积。结果AB-8型大孔吸附树脂纯化效果较好,最佳工艺条件为:上样液浓度为含生药量0.20g·mL-1,上样量为每1mL湿树脂上样0.375g生药,吸附速率为1mL·min-1,洗脱液为4倍柱体积的70%乙醇,洗脱速率为2mL·min-1;树脂经95%乙醇和1moL·L-1氢氧化钠溶液再生后,可重复使用3次。总黄酮与槲皮苷的保留率达70%、95%以上,峰1～3的保留率达90%以上,纯化后总黄酮及槲皮苷的含量分别提高了2.48倍和3.29倍。结论AB-8型大孔吸附树脂能较好地纯化富集侧柏叶总黄酮。Objective To study the process conditions of purifying the total flavonoids from Cacumen Platycladi by AB - 8 resin. Methods Spectrophotometric method was used to detect the total flavonoids, and HPLC was used to determine quercitrin and analyze the characteristic peaks of Fingerprint. Results The purification effect was satisfactory when the concentration of original solution of the extract of Cacumen Platycladi was 0.20 g ·mL^-1, the loading amount was 0. 375 g of Cacumen Platycladi per 1 mL of wet AB - 8 resin, the adsorption velocity was 1 mL ·min^-1, the eluant was 70 % alcohol being 4 times as much as the resin volume, and the elution velocity was 2 mL·min^-1. AB - 8 resin could be used for 3 times repeatedly after being reproduced by 95 % of ethanol and 1 moL·L^-1 of natrium hydroxydatum （NaOH）. The remaining rate of the total flavonoids and quercitrin was over 70 % and 95 % respectively, and the remaining rate of peaks 1 - 3 was over 90 %. After being purified by the AB - 8 resin, the contents of the total flavonoids and quercitrin were raised 2.48 times and 3.29 times more than those before purification respectively. Conclusion AB - 8 resin is fit for separating and purifying the total flavonoids from Cacumen Platycladi.

关 键 词：侧柏叶 总黄酮 槲皮苷 指纹图谱 大孔树脂吸附法 

分 类 号：R284[医药卫生—中药学] TQ460.6[医药卫生—中医学]