穿通支原体感染对肿瘤浸润及转移的影响  

The effect of Mycoplasma penetrans infection on tissue infiltration and metastasis of tumor

作　　者：朱丰村[1] 周丽萍[1] 

机构地区：[1]温州医学院检验学院,浙江温州325003

出　　处：《检验医学》2010年第2期113-117,共5页Laboratory Medicine

摘　　要：目的探讨穿通支原体(Mpe)感染对肿瘤浸润和转移的影响。方法收集53例膀胱移行细胞癌(TCCB)和40例直肠癌患者的手术切除组织标本和血液标本;以34例前列腺炎、膀胱炎及前列腺增生患者,21例直肠息肉患者的组织标本和血液标本及44名健康体检者血液标本作为对照。192例研究对象(共340份标本)分别进行Mpe分离培养鉴定,可疑阳性及阳性菌株用聚合酶链反应(PCR)予以核实。结果TCCB组Mpe累积分离率(43.4%,23/53)与前列腺炎组(17.6%,6/34)比较,差异有统计学意义(P<0.05);直肠癌组Mpe累积分离率(70.0%,28/40)与直肠息肉组(4.8%,1/21)比较,差异有统计学意义(P<0.01);肿瘤组Mpe总累积分离率(54.8%,51/93)与健康体检组(0.0%,0/44)比较,差异有统计学意义(P<0.01);Mpe阳性TCCB组组织浸润检出率与Mpe阴性组比较,差异有统计学意义(P<0.05);Mpe阳性直肠癌组组织浸润检出率与Mpe阴性组比较,差异有统计学意义(P<0.05);Mpe阳性直肠癌组淋巴结转移检出率与Mpe阴性组比较,差异有统计学意义(P<0.05)。结论穿通支原体感染与肿瘤组织浸润以及转移相关联。Objective To investigate the infection of Mycoplasma penetrans （Mpe） in patients with transitional cell carcinoma of bladder （TCCB） and patients with rectal carcinoma, and find out their relationship with tissue infiltration and metastasis of the tumor. Methods Blood and tissue specimens were collected from 93 carcinoma patients, including 53 cases of TCCB and 40 cases of rectal carcinoma. Meanwhile, there were 34 patients with prostatitis, cystitis and hyperplasia of prostate gland and 21patients with proctopolypus and 44 healthy subjects were performed as controls. The Mpe were isolated and cultured from these samples （total： 192 cases and 340 specimens） and the positive and suspected-positive isolates were confirmed by nested polymerase chain reaction（nPCR）. Results The rate of Mpe in TCCB group （43.4% ,23/53 ）was significantly higher than that in the prostatifis group（ 17.6%, 6/34, P 〈0.05 ）, and the rate in the rectal carcinoma group（70.0% ,28/40）was also significantly higher than that in the proctopolypus group （4.8% , 1/21, P 〈 0.01 ）. The total positive rate of Mpe （54.8% ,51/93 ） in tumor cases was significantly higher than that in the control group （0.0% , 0/44, P 〈0.01 ）. The detected rate of tissue infiltration in the Mpe infection group was significantly higher than that in non^infecfion group （ P 〈 0.05 ）. The detected rate of lymphatic metastasis of rectal carcinoma patients in Mpe infection group was significantly higher than that in non-infection group （ P 〈 0. 05 ）. Conclusions The result suggests that the tissue infiltration and metastasis of TCCB and rectal carcinoma are correlated to Mpe infection.

关 键 词：穿通支原体 肿瘤 组织浸润 淋巴结转移 

分 类 号：R375[医药卫生—病原生物学]