Chrom ID ESBL显色平板对产ESBLs肠杆菌科细菌筛选作用的评估  

Evaluation of ChromID ESBL medium for screening of ESBLs-producing Enterobacteriaceae

作　　者：韩立中[1] 杨海慧[2] 祝艳萍[2] 蒋晓飞[3] 张秀珍[4] 倪语星[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院,上海200025 [2]上海交通大学医学院附属仁济医院,上海200001 [3]复旦大学附属华山医院,上海200040 [4]卫生部北京医院,北京100730

出　　处：《检验医学》2010年第2期118-121,共4页Laboratory Medicine

基　　金：上海市自然科学基金项目(09ZR1419200);卫生部卫生公益性行业科研专项经费项目(200802107)

摘　　要：目的评估新的产色培养基Chrom ID ESBL在检测产超广谱β-内酰胺酶(ESBLs)肠杆菌科细菌中的可靠性。方法用2组肠杆菌科细菌进行评估,一组为285株上海地区检出的临床分离株,这些菌株已用美国临床实验室标准化研究所(CLSI)推荐的确证试验确认了是否产ESBLs,另一组28株产ESBLs肠杆菌科细菌,在以前的研究工作中已确认了这些菌株的产酶特性和ESBLs基因型别。结果Chrom ID ESBL能有效地检测产ESBLs的肠杆菌科细菌。结论Chrom ID ESBL是一种可靠的产ESBLs肠杆菌科细菌的筛选培养基。Objective To evaluate the reliability of a new selective ehromogenic agar medium, ChromlD ESBL, for deteetion of extended-speetrum beta-lactamases （ESBLs）-producing Enterobacteriaceae. Methods Two arrays of isolates were used to evaluate the reliability of ChromID ESBL. One array eontained 285 clinical isolates in which the ESBLs-producing feature was sereened by confirmatory tests recommended by Clinical and Laboratory Standards Institute （CLSI）. The other array contained 28 enterobacterial isolates with their ESBLs gene and producing enzyme feature being confirmed previously. Results Both groups of ESBLs-producing Enterobacteriaceae could be identified by ChromlD ESBL. Conclusions ChromID ESBL is a reliable culture medimn for screening and identifieation of ESBLs-produeing Enterobacteriaceae.

关 键 词：ChromID ESBL显色平板 超广谱Β-内酰胺酶 肠杆菌科 评估 

分 类 号：R446.5[医药卫生—诊断学]