流加发酵对重组Bacillus subtilis发酵生产角质酶的影响  

Effects of Fed-fermentation on Cutinase Production by Recombinant Bacillus subtilis

作　　者：陈晟[1,2] 张芙华[1,2] 陈坚[1,2] 吴敬[1,2] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122 [2]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《中国生物工程杂志》2010年第1期62-66,共5页China Biotechnology

基　　金：国家"863"计划(2009AA02Z204);教育部新世纪优秀人才支持计划(NCET-06-0486);食品科学与技术国家重点实验室科研基金(SKLF-MB-200802)资助项目

摘　　要：为实现基因工程菌Bacillus subtilis WSHB06-07生产角质酶的高产,在3L发酵罐中考察了不同初糖浓度对菌体生长和产酶的影响,并在选择38g/L初始蔗糖浓度的基础上,进行碳源的分批流加和恒速流加,结果表明发酵16h开始流加碳源,采用总补糖量60g/L,蔗糖平均流速为4g/(L.h)的恒速补料方式,角质酶酶活在31h可达到最大545.87U/ml,比分批发酵酶活提高67.8%,并获得较高的角质酶生产强度,满足工业化生产要求。In order to achieve the high production of cutinase by recombinant Bacillus subtilis WSHB06-07,the effect of various initial sucrose concentration on enzyme production was investigated in 3 L fermentor.It was found that sucrose concentration of 38 g/L is suitable for cell growth and cutinase production.Based on this sucrose concentration,fed-batch as well as constant-fed fermentation were investigated.The results showed that,using constant-fed fermentation from 16 h to 31 h and sucrose feed rate of 4 g/（L·h）,the cutinase activity in the culture media reached 545.87U/ml,which was increased by 67.8% compared to that of batch fermentation.By this way,high cutinase productivity was gained and it is beneficial for industrial production.

关 键 词：BACILLUS SUBTILIS 重组角质酶 分批流加发酵 连续流加发酵 碳源 生产强度 

分 类 号：Q789[生物学—分子生物学]