强直性脊柱炎患者外周血T细胞凋亡及凋亡相关蛋白的表达  

Expression of Apoptosis Related Proteins in Peripheral Blood T Cells in Ankylosing Spondylitis

作　　者：赵丹 杨小猛 苏卓娃[3] 马文松[3] 

机构地区：[1]深圳市罗湖区慢性病防治院,深圳518023 [2]深圳市罗湖区妇幼保健院,深圳518025 [3]深圳市南山区人民医院,深圳518052

出　　处：《热带医学杂志》2010年第1期45-47,F0002,共4页Journal of Tropical Medicine

基　　金：深圳市科技局科研计划项目(No.200405067)

摘　　要：目的探讨强直性脊柱炎患者外周血T细胞凋亡相关蛋白的表达以及T细胞凋亡状况,分析其在疾病免疫发病机制的作用。方法CT检查患者骶髂关节并分级。尼龙棉柱法分离患者外周血T细胞,PI染色结合流式细胞术检测T细胞凋亡率,免疫组化法检测T细胞促凋亡蛋白FADD、Caspase-3、Caspase-8和抗凋亡蛋白FLIP表达。结果与健康对照相比,强直性脊柱炎患者外周血T细胞凋亡率明显降低(P<0.05);促凋亡蛋白FADD、Caspase-3、Caspase-8百分率均明显降低(P<0.05),抗凋亡蛋白FLIP百分率明显升高(P<0.05)。结论强直性脊柱炎患者外周血T细胞凋亡蛋白及抗凋亡蛋白表达异常和T细胞凋亡不足与患者免疫学发病机制相关。Objective To investigate the role of apoptosis and apoptosis related proteins of peripheral blood T lymphocytes in ankylosing spondylitis（AS）. Methods The ankylosing spondylitis patients were diagnosed according to the 1984＇s diagnostic criteria of ankylosing spondylitis and divided into grade I , II and III based on computerized tomography（CT） result. Apoptotic ratios of T lymphocytes were evaluated by FCM. Expression of apoptosis related proteins of T lymphocytes including FADD, Caspase-3, Caspase-8 and FLIP were analyzed by immunohistochemical staining. Results The apoptotic ratios of peripheral blood T lymphocytes in AS were clearly lower than healthy control （P〈0.05）. The expression of pro-apoptotic proteins FADD, Caspase-3 and Caspase-8 were clearly lower than healthy control （P〈0.05）, while the expression of anti-apoptotic protein FLIP were obviously higher than healthy control （P〈0.05）. Conclusion Abnormal expression of apoptosis related proteins and low apoptosis of T lymphocytes in ankylosing spondylitis might involve in the immunopathogenesis of ankylosing spondylitis.

关 键 词：强直性脊柱炎 T细胞 促凋亡蛋白 抗凋亡蛋白 

分 类 号：R593.23[医药卫生—内科学] R392.11[医药卫生—临床医学]