提高水稻花培育种效率研究  

Study on Increasing Pollen Culture Breeding Efficiency in Rice

作　　者：苗立新[1] 李鑫[1] 谢丽霞[1] 佟彤[1] 何功志 王力嘉 

机构地区：[1]辽宁省盐碱地利用研究所,辽宁盘锦124010 [2]盘锦东禾种业有限公司,辽宁盘锦124010 [3]大石桥市茂洋种子有限公司,辽宁营口115000

出　　处：《北方水稻》2010年第1期15-17,37,共4页North Rice

摘　　要：通过对7个杂交组合F1代的花药培养,探讨了花培材料不同低温处理、不同诱导分化培养基激素配比、不同添加物以及培养条件对花药培养效率的影响,结果表明:低温预处理可提高花培的诱愈率,以8℃、8 d处理效果最好;改良N6培养基+YD2处理为粳稻、偏粳材料愈伤组织诱导最佳培养基,MS培养基+FH4处理为粳稻、偏粳材料愈伤组织分化的最佳培养基;多效唑(MET)对绿苗分化具有很大的促进作用;水解乳蛋白(LH)等有机附加物对多数材料的愈伤组织诱导和分化都有一定的作用;光、温培养条件对愈伤组织诱导分化有影响。The effect of pollen culture conditions on pollen culturing efficiency of rice was studied in the conditions of pre-low temperature treatment, different induction hormone formula, and additives with F1 of seven crosses as entries. The result showed that pre-low temperature, 8 ℃ for 8 d, was able to increase callus induction rate; modified N6＋YD2 medium was a suitable callus induction medium for japonica or partial japonica rice materials and MS＋FH4 medium was favorable for callus differentiation for japonica or partial japonica rice materials; MET promoted green seedling differentiation and lactoalbumin hydrolysate （LH） had effect on callus induction of most materials; light and temperature also affected callus induction and differentiation.

关 键 词：花培 愈伤组织诱导率 绿苗分化率 育种效率 

分 类 号：S511.035[农业科学—作物学]