高锰酸钾-甲醛-穿心莲内酯体系的流动注射化学发光分析法  

Flow Injection Chemiluminescence Determination of Andrographolide Using Potassium Permanganate and Formaldehyde System

作　　者：张慧[1] 林洁华[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《青岛科技大学学报（自然科学版）》2010年第1期37-41,共5页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(20775038);山东省自然科学基金项目(Q2007B03)

摘　　要：在酸性介质中，高锰酸钾能氧化穿心莲内酯发生化学发光反应，而甲醛的存在可使发光强度增强，在体系中加入表面活性剂十六烷基三甲基溴化铵可以进一步增强该体系的化学发光强度。据此建立了1种流动注射化学发光测定穿心莲内酯的新方法。方法的线性范围为5．0×10^-6～2．5×10^-4g·mL^-1，检测限1．0×10^-6g·mL^-1，相对标准偏差0．9％（n=12，C=1．0×10^-5g·mL^-1）。样品测量通量150h-1。该法用于药物中穿心莲内酯含量的测定，结果令人满意。同时，对高锰酸钾-甲醛-穿心莲内酯体系化学发光反应机理进行了探讨。为穿心莲内酯的快速定量测定提供了1种简便、高效的方法。A simple flow injection chemiluminescence procedure for the determination of andrographolide was proposed, which is based on the CL emitting reaction between andrographolide and potassium permanganate in acidic medium, greatly enhanced by form- aldehyde. Cetyl trimethyl ammonium bromide can further enhance the CL signal of the system. Under the optimized experimental conditions, andrographolide could be deter- mined in a linear range from 5.0×10^-6～2.5×10^-4g·mL^-1 with a detection limit of 1.0 × 10^-6 g·mL-1. The relative standard deviation of the proposed method calculated from 12 repetitive injections of 1.0 ×10^-5g·mL-1 andrographolide was 0.9%. The sample throughput was 150 h 1. The proposed method has been satisfactorily aplied to the determination of andrographolide in pharmaceutical formulations with no evidence of interference from common medicaments. In addition, the CL reaction mecha- nism of KMnO4-HCHO-AD system was also explored. The proposed method provided a strategy for rapid determination of andrographolide.

关 键 词：穿心莲内酯 化学发光 流动注射 高锰酸钾 甲醛 

分 类 号：O657.3[理学—分析化学]