微粒子酶免疫分析法和胶乳增强免疫比浊法测定β_2-微球蛋白的评价  

Assessment of Micro-particle Enzyme Immunoassay and Latex-enhanced Immunoturbidimetry for Measurement of β_2-microglobulin

作　　者：范玉平[1] 郭新荣[1] 郁超[1] 

机构地区：[1]南通市第一人民医院检验科,江苏南通226001

出　　处：《标记免疫分析与临床》2010年第1期41-44,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：对微粒子酶免疫分析法和胶乳增强免疫比浊法测定血清和尿液β2-微球蛋白(β2-MG)结果偏差进行评估。按照美国国家实验室标准委员会(NCCLS)EP9-A文件,以微粒子酶免疫分析法为实验方法,胶乳增强免疫比浊法为比较方法进行对比及偏差评估,将测定数据进行相关分析,并对两种分析系统之间的预期偏差进行评估。结果发现,除高浓度(>10mg/L)尿液样本外,其余各浓度的血清和尿液β2-MG用两种方法的测定结果的偏差均在可接受的范围。在使用性能较好的全自动生化分析仪和相配套试剂的前提下,微粒子酶免疫分析法和胶乳增强免疫比浊法对血清和尿液β2-MG的测定结果基本一致。To evaluate the deviation between micro-particle enzyme immunoassay and latex-enhanced immunoturbidimetry for measurement of β2-microglobulin in serum and urine,the β2-microglobulin in serum and urine samples were measured by micro-particle enzyme immunoassay and latex-enhanced immunoturbidimetry,and the correlation of measured data were analyzed to evaluate the prospective deviation between the two analytic systems according to the profile NCCLS-EP9-A.The results indicated that the deviation of measured results between the two analytic systems in all concentrations of serum and urine specimens were in the acceptable area except the high concentration（〉10mg/L） of urine specimens.Both micro-particle enzyme immunoassay and latex-enhanced immunoturbidimetry methods by automatic biochemical analyzer with the satisfying capability and good matching reagent could be used identically to measure the β2-microglobulin concentration in serum and urine.

关 键 词：Β2-微球蛋白 微粒子酶免疫分析法 胶乳增强免疫比浊法 偏差 

分 类 号：R446.6[医药卫生—诊断学]