豚鼠脑震荡后2～4周听功能与耳蜗扫描电镜的改变  

CHANGES IN AUDITORY FUNCTION AND COCHLEAR ULTRASTRUCTURE UNDER SCANNING ELECTRON MICROSCOPE AT 2～4 WEEKS AFTER CEREBRAL CONCUSSION IN GUINEA PIGS

作　　者：张颖[1] 路虹[1,2] 王文利[1] 韩海霞[1] 张喜琴[1] 段茂利[2] 

机构地区：[1]河北医科大学第二医院耳鼻咽喉科,河北石家庄050000 [2]卡罗琳斯卡医学院耳鼻喉头颈外科,斯德哥尔摩17176

出　　处：《河北医科大学学报》2010年第2期168-171,共4页Journal of Hebei Medical University

基　　金：河北省科技支撑计划项目(08276101D-35)

摘　　要：目的观察脑震荡后2～4周听功能及耳蜗毛细胞的病理改变。方法以豚鼠为研究对象,制成脑震荡模型。20只健康豚鼠随机分为4组,正常对照组,脑震荡后即刻组、2周组、4周组。应用听性脑干反应(auditorybrainstem response,ABR)和畸变产物耳声发射(distortion products otoacoustic emissions,DPOAE)观察脑震荡后听功能变化;应用扫描电镜观察耳蜗毛细胞的病理改变。结果 ABR Ⅰ、Ⅴ波潜伏期和Ⅱ～Ⅲ、Ⅱ～Ⅴ波间期均有变化;DPOAE于打击后在0.7、1.0 kHz频率其幅值有明显变化(P<0.05);扫描电镜下见耳蜗毛细胞的纤毛有排列紊乱和倒伏现象。结论豚鼠脑震荡后2～4周听功能和耳蜗毛细胞均存在一定程度的病理改变。Objective To observe the auditory function and the pathological changes of cochlear hair cells at 2-4 weeks after cerebral concussion. Methods Twenty healthy guinea pigs were divided into four groups. Auditory brainstem response （ABR） and distortion products otoacoustic emissions （DPOAE） were recorded at immediately, 2 weeks and 4 weeks after concussion. Brain and cochlear structure were observed under light microscope and scanning electron microscope. Results ABR latency of Ⅰ,Ⅴ wave and Ⅱ～Ⅲ,Ⅱ～Ⅴ interval waves were changed after concussion. DPOAE were changed significantly at 0.7 and 1 kHz after concussion. The disarranged and fallen hair cells were shown under scanning electron microscope. Conclusion There were some changes in auditory function and cochlear structure at 2-4 weeks after cerebral concussion.

关 键 词：脑震荡 耳声发射 自发性 豚鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科]