耐甲氧西林金黄色葡萄球菌的耐药性及消毒剂耐药基因的检测  

DETECTION OF THE DRUG RESISTANCE OF MRSA AND ITS DISINFECTANT-RESISTANT GENE

作　　者：孙静娜[1] 张征[1] 武艳[1] 张卫国[2] 高志成[3] 代丽丽[1] 

机构地区：[1]河北医科大学第一医院检验科,河北石家庄050031 [2]河北医科大学期刊社,河北石家庄050017 [3]保定市第二医院药剂科,河北保定071051

出　　处：《河北医科大学学报》2010年第1期66-68,共3页Journal of Hebei Medical University

基　　金：河北省科学技术研究与发展指导计划(052761136);河北省医学科学研究重点课题计划(06197)

摘　　要：目的了解耐甲氧西林金黄色葡萄球菌(methicillin resistant staphylococcus aureus,MRSA)对抗菌药物的耐药性和产生耐消毒剂基因的情况。方法收集2007年1月—2008年6月分离自河北医科大学第一医院临床患者的MRSA 45株,采用Kerby-Bauer纸片扩散法测定细菌对17种抗生素的敏感性;采用聚合酶链式反应对45株MRSA的消毒剂耐药基因qacA进行检测。结果 MRSA对青霉素G、红霉素、克林霉素、环丙沙星、四环素的耐药率均在70%以上,消毒剂耐药基因qacA的检出率为40%。结论 MRSA呈多重耐药,消毒剂耐药基因qacA检出率较高,是MRSA对消毒剂耐药的重要机制。Objective To understand the drug resistance of methicillin-resistant staphylococcus aureus （MRSA） to the antibacterials and the disinfectant-resistant gene. Methods Forty-five strains of MRSA were collected from January 2007 to June 2008 in the first hospital of Hebei medical university. Kerby-Bauer method was used to detect the sensitivity of the bacteria to 17 kinds of antibiotics; QacA gene, one of the disinfectant-resistant gene was determined by polymerase chain reaction （PCR） in all 45 straing of MRSA. Results The resistance rate of the MRSA to penicillin, gerythromycin, clindamycin, ciprofloxacin, tetracycline was all above 70%, the detection rate of disinfectant-resistant gene qacA was 40%. Conclusion MRSA is multidrug resistant, the detection rate of qacA is high,which is an important mechanism of the drug resistance of MRSA.

关 键 词：球菌 金黄色葡萄 基因 聚合酶链反应 

分 类 号：R378.1[医药卫生—病原生物学]