松褐天牛成虫高毒力绿僵菌菌株筛选及液体振荡培养条件  

Studies on the Screening and Liquid Shake Culture Condition of High Virulent Metarhizium anisopliae against Monochamus alternatus Hope Adults

作　　者：潘永胜[1,2] 徐福元[2] 韩正敏[3] 刘云鹏[2] 

机构地区：[1]江苏省宿迁市林业局,江苏宿迁223800 [2]江苏省林业科学研究院,江苏南京211153 [3]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《林业科学研究》2010年第1期102-107,共6页Forest Research

基　　金：松材线虫病生物治理技术的推广应用(国家林业局科技成果推广项目编号:[2006]21号)

摘　　要：对3株绿僵菌菌株通过不同温度水浴处理,发现了孢子的萌发条件;并开展了3菌株对松褐天牛成虫的毒力测定,筛选出M a789菌株的致病力较强。通过正交试验对其液体振荡培养条件进行了分析,选出了菌丝体生长最适培养基为蔗糖20 g.L-1,酵母粉10 g.L-1,KH2PO43.0 g.L-1,MgSO4.7H2O 1.0 g.L-1,CaC l20.5 g.L-1;培养液生孢子最适培养基为蔗糖20 g.L-1,蛋白胨10 g.L-1,KH2PO41.5 g.L-1,MgSO4.7H2O 2.0 g.L-1,CaC l20.5g.L-1。The germination percentage with different temperatures water bath treatment and virulent against Monochamus alternatus of different Metarhizium anisopliae were measured in this paper. The results showed that Metarhizium anisopliae789 （ Ma789 ） was the most virulent one. Conditions of liquid shake culture to Ma789 were studied by orthogonal test, the optimum liquid medium recipe was sucrose 20g·L^-1, yeast-extract paste 10 g·L^-1, KH2PO4 3.0 g·L^-1 ,MgSO4 · 7H2O 1.0 g·L^-1 ,CaCl2 0.5 g·L^-1 for mycelium culture; but for yielding spore, the optimum liquid medium recipe was sucrose 20g·L^-1, peptone 10 g·L^-1 ,KH2PO4 1.5 g·L^-1,MgSO4 ·7H2O 2.0g·L^-1,CaCl2 0.5 g·L^-1.

关 键 词：金龟子绿僵菌 松褐天牛 毒力测定 液体振荡培养 

分 类 号：S763[农业科学—森林保护学]