光对胆红素稳定性的影响及其在桂蒲肾清片中的含量测定  

Effect of light on stability of cholerythrin and content of cholerythrin in the Guipu Shenqing tablet

作　　者：张东方[1] 周美环 袁长季[1] 

机构地区：[1]中国医科大学药学院生药教研室,沈阳110001 [2]沈阳东北大药房连锁店,沈阳110023

出　　处：《天津中医药》2010年第1期69-70,共2页Tianjin Journal of Traditional Chinese Medicine

摘　　要：[目的]考察胆红素的稳定性,为桂蒲肾清片中胆红素的含量测定方法提供依据。[方法]采用高效液相色谱(HPLC)法测定胆红素的含量,并进行了方法学考察;通过紫外光谱法和HPLC法测定胆红素日光照射前后的变化情况。[结果]胆红素不稳定,在避光的条件下,0.5h峰面积即下降1.6%,但方法学考查表明:精密度(RSD=1.2%)、重复性(RSD=2.0%)及回收率(96.0%,RSD=0.8%)可满足实验要求;胆红素对光非常敏感,日光下3h后紫外光谱完全变化,通过液相色谱未能检出胆红素。[结论]HPLC法可以作为胆红素的测定方法,但应注意避光操作和快速测定。[Objective] To investigate the stability of cholerythrin and measure its content in the Guipu Shenqing tablet. [Methods] Cholerythrin was determined by HPLC. The amount of cholerythrin before and after the explosion of sunlight was compared by HPLC and UV spectrum. [Results] Cholerythrin was not stable and its peak area decreases for 1.6% in half an hour without light. It showed that the precision （RSD=1.2%）, reproducibility （RSD=2.0%） and recoveryrate couldreach the need of experiments. Cholerythrin was sensitive to sunlight. It completely vanishes in sunlight for 3 hours and no cholerythrin was found by HPLC. [Conclusion] HPLC could be used to measure the content of cholerythion and the drug should be prepared quickly with no light.

关 键 词：HPLC 胆红素 稳定性 

分 类 号：R284.1[医药卫生—中药学]