骨髓间充质干细胞对大鼠实验性肺气肿的作用  

Effect of mesenchymal stem cells on experimental emphysema of rat models

作　　者：李旭[1] 李宝平[1] 

机构地区：[1]山西医科大学,山西太原030001

出　　处：《中国医药导报》2010年第3期11-13,共3页China Medical Herald

摘　　要：目的:观察骨髓间充质干细胞(MSCs)对肺气肿的治疗作用。方法:Wistar大鼠随机分为四组,分别为健康组(A组)、模型组(B组)、生理盐水对照组(C组)和治疗组(D组)。B、C、D组均利用烟熏的方法诱导其肺气肿的形成,之后D组采用DAPI标记的骨髓间充质干细胞进行治疗。结果:4周后取肺组织做病理切片,观察肺泡变化,进行肺泡计数,并计算单位面积平均肺泡数(MNa)和平均肺泡面积(MAA)。D组单位面积平均肺泡数明显高于B组(P<0.05)和C组(P<0.05),但低于A组(P<0.05),B组和C组之间无显著性差异(P>0.05);B组和D组平均肺泡面积无显著性差异(P>0.05),B组和C组之间无显著性差异(P>0.05),D组平均肺泡面积明显低于B组(P<0.05)和C组(P<0.05)。MSCs治疗组大鼠肺组织中可见DAPI标记的细胞,并且观察到MSCs在受体肺组织内分化为上皮细胞。结论:经骨髓间充质干细胞治疗的肺气肿模型大鼠的肺泡数较B组和C组明显增加,平均肺泡面积明显减小,且MSCs在肺气肿模型大鼠肺组织中分化为肺泡上皮,说明骨髓间充质干细胞对治疗大鼠实验性肺气肿是有效的。Objective：To investigate the therapeutic effect of mesenchymal stem cells on emphysema.Methods：Wistar rats were randomly divided into four groups,normal group （group A）,model group （group B）,NS control group （group C） and treatment group （group D）.Experimental emphysema rat models were made by smog in group B,C and D.MSCs were inject ed into recipient rats of group D after labeled with DAPI.Results：The alveoli were observed and counted with pathological section after 4 weeks,and MNa and MAA were calculated.The MNa of group D was significantly more than that of group B （P〈0.05） and group C （P〈0.05）,but it was less than that of group A （P〈0.05）.There was no significant difference in MNa between group B and group C （P〉0.05）.There was no significant difference in MAA between group A and group D （P〉0.05）,there was no significant difference in MAA between group B and group C （P〉0.05） either.The MAA of group D was significantly smaller than that of group B （P〈0.05） and group C （P〈0.05）.The MSCs labeled with DAPI were found in the lungs of rats of treatment group.It was found that MSCs differentiated into alveolar epithelial cells in the lungs of acceptor.Conclusion：By injecting MSCs into the veins of treat experimental emphysema,the MNa of group D is more than that of group B and group C.The MAA of the treatment group is significantly reduced,and MSCs can differentiate into alveolar epithelial cells.It indicates that MSCs are effective to the experimental emphysema treatment.

关 键 词：骨髓间充质干细胞 肺气肿 动物模型 治疗 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]