腰腿理痛散对硬膜外移植自体髓核大鼠背根节中P物质的影响  

EFFECTS OF YAOTUI LITONG POWDER ON SUBSTANCE P CHANGES IN DORSAL ROOT GANGLION OF RATS WITH AUTOGRAFTING NUCLEUS PULPOSUS TO CAVUM EPIDURALE

作　　者：张思胜[1] 潘建西[1] 谈明顺 王临青 

机构地区：[1]甘肃省兰州市城关区中医骨伤科医院,兰州730010 [2]甘肃省兰州市西固区中医院,兰州730060

出　　处：《中国中医药科技》2010年第1期17-18,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：甘肃省中医药科研项目No.05-GZK-L-01

摘　　要：目的:观察腰腿理痛散对硬膜外移植自体髓核大鼠背根节(DRG)中P物质(SP)的影响。方法:取健康Wistar大鼠50只,随机分为假手术组、模型组、腰痛宁组、腰腿理痛散高剂量组、腰腿理痛散低剂量组,每组10只,将大鼠自身的尾椎髓核取出移植于左侧硬膜外腔L5、L6神经根背侧,造成非压迫性大鼠自体髓核移植硬膜外模型,通过免疫组化的方法测定大鼠2周时DRG中SP的变化。结果:造模各组DRG中SP阳性神经元的表达明显增加,免疫反应强度均增高,与假手术组比较P<0.05～0.01;各用药组较模型组明显降低(P<0.05),腰腿理痛散组较阳性组明显降低(P<0.05)。结论:腰腿理痛散可通过下调或者抑制非机械压迫性髓核对神经根损伤后DRG内的痛觉神经递质SP的释放而发挥镇痛作用。Objective: To observe the effects of yaotui litong powder on substance P changes in dorsal root ganglion of rats with autografting nucleus pulposus to cavum epidrale. Methods: Rats were randomly divided into five groups, the sham operated,model, Yaotongning contol, yaotui litong powder high dose and low dose groups, each group contained 10 rats. The non-compressive models with transplanted autogenous nucleus pulposus were made by removing autogenous nucleus pulposus from the coccygeal vertebral and placing it into the back of L5～6 nerve roots of left cavum epidurale. Each group was given corresponding management, substance P changes in dorsal root ganglion of each group were determined by immunohistochemistry method after modeling two weeks.Results: To compare with the sham operated group, the number of SP positive neurons in dorsal root ganglion of each modeling group rats was obviously increased,and the immunoreaction intensity of SP positive neurons was enhanced. To compare with the model group, the number and immunoreaction intensity of SP positive neurons in dorsal root ganglion of each treated group rats were lowered significantly(P<0.05), the effects in yaotui litong powder high and low dose groups were more obvious than those in positive group.Conclusion: Yaotui litong powder has obvious analgasic effect by down-regulating or inhibiting SP expression of dorsal root ganglion after nerve root injury from non-compressive nucleus pulposus protrusion.

关 键 词：椎间盘移位/中医药疗法 @腰腿理痛散/治疗应用 神经递质 大鼠 

分 类 号：R285.5[医药卫生—中药学]