亚砷酸钠对G361细胞ROS水平及TYR影响  

Effects of sodium arsenite on ROS level and TYR activity in G361 cells

作　　者：李昕[1] 李冰[1] 侯萍[2] 张新玉[1] 朱博[1] 张森佳[2] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室,沈阳110001 [2]中国医科大学

出　　处：《中国公共卫生》2010年第1期20-21,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30400362)

摘　　要：目的研究亚砷酸钠(NaAsO2)对人类黑色素瘤G361细胞活性氧自由基(ROS)水平及酪氨酸酶(TYR)活力的影响。方法将体外培养的人类黑色素瘤G361细胞分别暴露于NaAsO2浓度为0.1,0.5及5.0μmol/L2及6h后,测定细胞内ROS水平及TYR活力。结果NaAsO25μmol/L2及6h〔(130.12±3.07)%〕〔(139.55±11.02)%〕染毒组ROS水平明显高于各相应染毒时段对照组水平;染毒2及6h各剂量组TYR活力分别为(99.31±0.81)%,(99.85±1.34)%,(99.52±1.43)%和(99.50±1.88)%,(100.35±1.95)%,(101.68±1.66)%,与各自对照组比较,差异无统计学意义。结论NaAsO2作用下,随着染毒剂量增高,G361细胞内ROS的生成水平增多,呈剂量-效应关系。Objective To study the effects of sodium arsenite on ROS level and TYR activity in G361 cells.Methods Human melanoma G361 cells were cultured with sodium arsenite at the concentration of 0.1,0.5,and 5.0 μmol/L for 2 and 6 hours,respectively.Cells were then measured for the levels of reactive oxygen species（ROS）and the actevities of tyrosinase（TYR）.Results ROS levels of the 5 μmol/L for 2 hr（130.12±3.07%） and for 6 hr（139.55±11.02%） exposure group were significantly higher than that of control group.TYR activity was 99.31±0.81,99.85±1.34,99.52±1.43% for 2 hr guoup and 99.50±1.88,100.35±1.95,101.68±1.66% for 6 hr group,respeetively.There was no significant difference between arsenic exposed and control guoups in TYR activity.Conclusion ROS levels increase with the concentration of sodium arsenic exposure in a dose dependent manner.

关 键 词：亚砷酸钠 活性氧自由基 酪氨酸酶 

分 类 号：R995[医药卫生—毒理学]