水黄皮根总黄酮抗大鼠幽门结扎型胃溃疡作用  

Effect of flavonoids from Pongamia pinnata root on gastric ulcer induced by pyloric-ligation in rats

作　　者：刘可云[1] 朱毅[2] 

机构地区：[1]湖北民族学院医学院生理学教研室,湖北恩施445000 [2]海南省药品检验所

出　　处：《中国公共卫生》2010年第1期78-80,共3页Chinese Journal of Public Health

基　　金：海南省自然科学基金(30530)

摘　　要：目的研究水黄皮根总黄酮(PRF)对大鼠幽门结扎型胃溃疡的作用机制。方法采用大鼠幽门结扎模型观察PRF对胃溃疡的保护作用;幽门结扎收集胃液观察PRF对大鼠胃液分泌和黏液分泌的影响;采用免疫组化染色法观察PRF对幽门结扎大鼠胃黏膜表皮生长因子(EGF)表达的影响;采用放射免疫方法观察PEF对幽门结扎大鼠血清胃泌素的影响。结果与模型对照组的溃疡指数(4.37±0.69)比较,PRF中、高剂量组分别为(2.62±0.69),(2.12±0.92),对大鼠幽门结扎型胃溃疡有明显的保护作用(P<0.01);与模型对照组的积分吸光度(0.67±0.24)比较,PRF低、中、高剂量组分别为(0.93±0.41),(1.36±0.59),(1.54±0.68),差异均有统计学意义(P<0.05,P<0.01);与模型对照组比较,PRF各剂量组可明显降低胃液量(P<0.05,P<0.01);PRF中、高剂量能明显降低大鼠游离酸度、总酸度以及总酸排出量(P<0.01)。结论水黄皮根总黄酮对大鼠幽门结扎型胃溃疡具有明显保护作用,可能与通过抑制胃泌素分泌、促进EGF分泌、促进胃黏液分泌有关。Objective To study the effect and mechanism of flavonoids from Pongamina pinnata roots （RPF） on gastic ulcer induced by pyloric-ligation in rats.Methods The anti-gastric ulcer effect of PRF was observed with gastic ulcer induced by pyloric-ligation.The effcets of PRF on gastric secretion and mucus secretion in rats were studied with pyloric-ligation.The expression of epidermal growth factor （EGF） was detected by immunohistochemistry.The contents of gastrin in pyloric-ligation rats were determined by radioimmunoassay.Results The index of gastric ulcer of model was 4.37±0.69.The medium and high dosage of PRF inhibited markedly gastric ulcer induced by pyloric-ligation wtih inhibition ratios of 40.04% and 51.49%,respectively（P0.01）.The mild and high dosage of PRF also obviously inhibited secretion of gastric acid and promoted the secretion of gastric mucus（P0.01）.Compared with the control guoup,the medium and high dosage PRF group showed decreased serum gastrin（P0.05,P0.01）.Conclusion PRF may inhibit gastric ulcer indued by pyloric-ligation.The mechanism of the effect might be associated with the decreased secretion of gastrin and increased expression of EGF,which inhibits the secretion of gastric acid and promots the secretion of gastric mucus.

关 键 词：水黄皮根总黄酮 幽门结扎型胃溃疡 胃酸分泌 黏酸分泌 胃泌素 表皮生长因子 

分 类 号：R28[医药卫生—中药学]