一株中度嗜盐细菌whb45的鉴定及其抗菌与抗肿瘤活性筛选  

Identification of a Moderately Halophilic Bacterium whb45 and Screening of Its Antimicrobial and Antitumor Activity

作　　者：陈雷[1,2,3] 王光玉[2] 卜同[1,3] 张允斌[2] 刘明[1] 张军 林秀坤[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071 [2]哈尔滨工业大学威海海洋学院,山东威海264209 [3]中国科学院研究生院,北京100039 [4]山东省医药丁业设计院,山东济南250013

出　　处：《微生物学通报》2010年第1期85-90,共6页Microbiology China

基　　金：国家863计划项目(No2007AA091403,2007AA09Z408)

摘　　要：从盐场中分离鉴定中度嗜盐细菌并对其潜在的抗菌和抗肿瘤活性进行评价。从山东威海的鹿道口盐场分离嗜盐细菌,对菌株whb45进行形态学和生理生化特性研究,测定其16SrRNA序列并通过同源性比对进行系统发育分析,采用抗菌和细胞毒模型进行活性筛选。试验结果表明,菌株whb45为中度嗜盐细菌,whb45与Halobacillus trueperi在形态和生理生化特征方面最接近,16SrRNA序列相似性为99%。whb45的粗提物对多种细菌、真菌和肿瘤细胞的生长都具有较强的抑制作用,可以作为发现生物活性物质的潜在的新来源。The aim of this study was to isolate and identify moderately halophilic bacteria from solar saltern and to investigate their potential antimicrobial and antitumor activities. Halophilic bacteria were isolated from Ludaokou Solar Saltem in Weihai of Shandong Province. The identification of the strain whb45 was determined by their morphological characteristics, physiological and biochemical tests and phylogenetic analysis based on 16S rRNA sequence comparison. The antimicrobial activity and cytotoxic activity were screened. Our results showed that the strain whb45 was belonged to moderately halophilic bacterium. Phylogenetic analysis showed the strain whb45 had a 99% homology with Halobacillus trueperi. The crude ex-tract from whb45 showed stronger antimicrobial activities which could inhibit the growth of several bacteria and fungi. MTT analysis revealed that the strain possessed potent anticancer activity with the stronger inhibitory effect on several human cancer cells. Our results suggested that the moderately halophilic bacterium whb45 may be developed as a potential new source for the discovery of novel bioactive substances.

关 键 词：中度嗜盐细菌 鉴定 喜盐芽孢杆菌属 抗菌活性 抗肿瘤活性 

分 类 号：Q93[生物学—微生物学]