胍丁胺干预对变态反应性脑脊髓炎的胶质纤维酸性蛋白表达、γ-干扰素和白介素-10水平影响的实验研究  

Experimental study of agmatine on the expression of glial fibrillary acidic protein and the levels of IFN-γ and IL-10 of allergic encephalomyelitis in rats

作　　者：潘大津 郑荣远[2,3] 邱伟文[4] 吴绍长 

机构地区：[1]丽水市第二人民医院老年病科,浙江丽水323000 [2]温州医学院附属第一医院神经内科,浙江温州325000 [3]温州医学院实验神经生物研究所,浙江温州325000 [4]温州医学院附属第五医院神经内科,浙江丽水323000

出　　处：《中国临床药理学杂志》2010年第1期44-48,共5页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省自然基金资助项目(Y204133)

摘　　要：目的观察胍丁胺(神经递质,抗炎药)对实验性变态反应性脑脊髓炎中枢神经系统内胶质纤维酸性蛋白表达及γ-干扰素和白介素-10水平的影响。方法用GPSCH免疫Wistar大鼠建立实验性变态反应性脑脊髓炎动物模型,于免疫当日,开始分别予25,50,100 mg.kg-1胍丁胺,腹腔注射,每天1次,连续14 d。比较各组行为学变化,用免疫组化法观察脑和脊髓组织中的胶质纤维酸性蛋白的表达;用双抗夹心ELISA法测定实验性变态反应性脑脊髓炎大鼠脑和脊髓组织内细胞因子γ-干扰素和白介素-10的水平。结果与模型组比较,胍丁胺给药14 d可明显降低变态反应性脑脊髓炎大鼠的发病率,改善其临床症状;脑和脊髓组织中,胶质纤维酸性蛋白表达增高(P<0.05),γ-干扰素水平降低和白介素-10水平升高(P<0.05或<0.01)。结论胍丁胺能促进胶质纤维酸性蛋白表达,调节实验性变态反应性脑脊髓炎中的Th1/Th2比例平衡,降低γ-干扰素,升高白介素-10。Objective To observe the effects of agmatine on the expression of glial fibrillary acidic protein （GFAP） and the levels of IFN-γ and IL-10 in experimental allergic encephalomyelitis （EAE） in rats. Methods EAE was induced in Wistar rats by immunization of spinal cords homogenate of guinea pigs and complete Freund＇s adjuvant. Rats gived with agmatine was intraperitoneally injected with agmtine at dosage of 25 ,50 , 100 mg·kg^-1, respectively, once daily for fourteen days after immunization. The ethologic change is observed, and the number of GFAP in central nervous system（CNS） was examined with the method of immunohistochemistry and the levels of IFN-γ and IL-10 of the CNS in EAE were observed with the method of sandwich-enzyme-linked inmunosorbent assay. Resuts Compared with EAE model rats, agmatine treatment significantly caused the decrease in morbidity and the improvement clinical manifestation of rats with EAE. The number of GFAP in and around the inflamatory demylinatve lession of CNS in EAE increased obviously （P 〈 0.05 ） , and the levels of IL-10 had significantly increased and the levels of IFN-γ had significantly decreased, compared with model group （ P 〈 0. 05 or 〈 0. 01 ）. Conclusion Agmatine could promote the expression of GFAP of central nervous system in EAE, regulate the ratio of Th1/Th2 in EAE, increased the levels of IL-10 and the levels of IFN-γ in the CNS of EAE.

关 键 词：变态反应性脑脊髓炎 胍丁胺 胶质纤维酸性蛋白 

分 类 号：R965.1[医药卫生—药理学] R976[医药卫生—药学]