毛竹根际分离的地衣芽孢杆菌固氮特性研究  

Studies on Nitrogen fixation Characters of Several Bacillus licheniformis Strains from Phyllostachys pubescens Roots

作　　者：顾小平[1] 吴晓丽[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《竹子研究汇刊》1998年第4期59-63,共5页Journal of Bamboo Research

基　　金：浙江省自然科学基金

摘　　要：从毛竹根际分离到36株固氮菌，经初步镜检其中17株菌为芽孢杆菌，进一步鉴定确定其主要为多粘芽孢杆菌（Bacilluspolymyxa）和地衣芽孢杆菌（Bacilluslicheniformis），本文主要就其中菌号为G－8、G－12、G－14、G－17四株地衣芽孢杆菌的鉴定及固氮特性进行研究。结果显示：四株菌均为杆状，具芽孢孢孢囊稍膨大，它们的生理生化特性相同，但菌落形态有一些差异。G－8和G－17的最佳固氮温度为35℃左右，G－12和G－14的最佳固氮温度为30℃左右；G－12、G－8和G－14的最适固氮pH为6．0左右，G－17的最适固氮pH为6.0～7.5；四株菌都可利用碳源固氮，但均以葡萄糖为碳源时的固氮活性最高，依次为64．76、437．23、65．42、90．91nmolC2H4h-1，瓶-1。G－12在培养48h，G－8、G－14在培养96h，G－17在培养60h的固氮活性最高分别为138．50、122．01、170．87、109．81nmolC2H4．h-1.瓶-1。Four strains: G-8, G-12, G-14 , and G-17 isolated from Phyllostachys pubescens roots are identified and studied. According to the research on morphology,physiological and biochemical character,these strains all belong to Bacillus licheniformis. Their optimum temperature on nitrogen fixing is 35 ℃ for G-8 and G-17, 30℃　for G-12 and G-14,optimum pH on nitrogen fixing is 6. 5 for G-8,G-12 and G-14, 6. 0 - 7. 5 for G-17. Many carbon sources can be used by these four strains,but the highest nitrogenase activites can be obtained when the carbon source is glucose, they are 64. 76, 437. 23, 65. 42, 90. 91 nmolC2H4. h-1. bottle-1 respectively. Under different culture time condition the highest nitrogenase activities are 122. 01, 170. 87 nmolC2H4. h-1. bottle-1 for G-8 and G-14 in 96h, 109. 81 nmolC2H4. h-1.bottle-1 for G-17 in 60h, 138. 50 nmolC2H4. h-1. bottle-1 for G-12 in 48h.

关 键 词：毛竹 芽孢杆菌属 地衣芽孢杆菌 固氮活性 

分 类 号：S795.720.1[农业科学—林木遗传育种] S718.521.3[农业科学—林学]