γ-聚谷氨酸生产菌的选育及培养条件研究  

Screening and optimizing culture conditions of Bacillus strain for producing γ-polyglutamic acid

作　　者：冯志彬[1] 程仕伟[1] 缪静[1] 张玉香[1] 王芹芹[1] 杨在东[1] 朱应普[1] 

机构地区：[1]鲁东大学生命科学学院,烟台264025

出　　处：《生物加工过程》2010年第1期40-44,共5页Chinese Journal of Bioprocess Engineering

基　　金：山东省教育厅科技计划资助项目(J08LE55);鲁东大学学科建设经费资助项目(203-07000108)

摘　　要：从土壤中筛选分离到1株γ聚谷氨酸的生产菌株yt102,初步鉴定为枯草芽孢杆菌;以此为出发菌株采用紫外线(UV)、亚硝基胍(NTG)进行复合诱变,获得1株γ聚谷氨酸高产突变株,突变株连续传代10次,发酵性能稳定;通过单因素和正交试验确定培养基的最佳组成,在最优条件下,γ聚谷氨酸的平均产量可达28.5 g/L。γ--Polyglutamic acid producing strain ytl02 was isolated from soils and taxonomically identified as Bacillus subtilis. A high productive strain of γ-polyglutamie acid was obtained from ytl02 with muta- genesis by UV and NTG, and it was genetic ally stable in 10 generations. Fermentation medium composi- tion for the mutant strain was optimized by a single factor and preliminary orthogonal experiments. The average yield of T-polyglutamic acid reached 28.5 g/L on the optimized medium.

关 键 词：枯草芽孢杆菌 优化培养 Γ-聚谷氨酸 筛选 

分 类 号：Q935[生物学—微生物学]