利用傅立叶红外光谱和原子力显微镜研究超声提取对鸡腿菇多糖结构的影响  

Effect of ultrasonic-assisted extraction on polysaccharide structure from Coprinus comatus characterized by FTIR and AFM

作　　者：刘娜女[1] 张静[2] 李岱[1] 徐平平[1] 王博[1] 孙润广[1] 

机构地区：[1]陕西师范大学物理学与信息技术学院生物物理与生物医学工程实验室,西安710062 [2]陕西师范大学食品工程与营养科学学院,西安710062

出　　处：《生物加工过程》2010年第1期56-60,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(10874108);陕西省自然科学基础研究计划资助项目(SJ08A16)

摘　　要：利用沸水浴法和超声辅助提取法提取鸡腿菇粗多糖,分别得到对应的多糖水浴提取的多糖(WCP)和超声提取的多糖(UCP)。采用苯酚硫酸法测得WCP和UCP中的总糖质量分数分别为87.997%和72.937%。用季铵盐沉淀法和Sephadex G 200凝胶层析法对WCP和UCP进行分离纯化,得WCP3 1、UCP3 1 2个主要组分,用傅立叶红外光谱(FTIR)和原子力显微镜(AFM)对提取多糖的结构进行表征。结果显示:WCP3 1和UCP3 1均具有一般多糖类物质的特征吸收峰,WCP3 1和UCP3 1的单糖残基均以β吡喃环存在,但超声波可导致部分C O双键断裂形成C—O链接;WCP3 1呈现大量的近似螺旋聚集体和少量球状聚集体,但是UCP3 1是较小的分散球状聚集体,说明超声波可断裂多糖的分子链间或链内氢键,导致近似螺旋聚集体的降解。Polysaccharides were extracted from Coprinus comatus by boiling water process and ultrasonic- assisted process, the obtained polysaccharides were designated as WCP and UCP, respectively. The total sugars of WCP and UCP were 89. 997% and 72. 937% determined by phenol-sulfuric acid method. The crude polysaccharides were purified by CTAB and Sephadex G-200 in sequence, and the obtained ones were designated as WCP3-1 and UCP3-1. FTIR showed that there existed β-pyranoside in the polysaccha- rides and AFM showed that WCP3-1 on mica existed in spiral and spherical form, but UCP3-1 existed in spherical form. The ultrasonic collapse of cavitation bubbles generated intense shock waves that damaged the intrachain or interchain hydrogen bonds.

关 键 词：鸣腿菇 超声提取 多糖 

分 类 号：Q336[生物学—遗传学]