金叶败毒制剂对人巨细胞病毒感染人胚肺成纤维细胞增殖及凋亡的影响  

Effect of Jinyebaidu preparation on proliferation and apoptosis of human embryonic lung fibroblast cells infected by human cytomegalovirus

作　　者：袁慧[1] 刘璐[2] 闻良珍[3] 

机构地区：[1]江汉大学医学院,湖北武汉430056 [2]华中科技大学同济医学院附属同济医院药剂科 [3]华中科技大学同济医学院附属同济医院妇产科

出　　处：《中国妇幼保健》2010年第3期390-392,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助项目(30271673);湖北省自然科学基金资助项目(2007ABA267);湖北省教育厅资助项目(B200734001)

摘　　要：目的:探讨中药金叶败毒制剂(简称中药)对人巨细胞病毒(HCMV)感染人胚肺成纤维细胞(HEL)增殖及凋亡的影响。方法:用HCMVAD169感染人胚肺成纤维细胞,分别用金叶败毒制剂和更昔洛韦(GCV)干预后,采用MTT法检测HCMV感染及药物干预后HEL细胞的增殖活性,并采用流式细胞术检测HEL、HCMV感染及药物干预后细胞凋亡指数。结果:在HCMV感染48h内,细胞增殖及凋亡指数与细胞对照组无明显差异。HCMV感染72h后,细胞增殖明显受抑制,凋亡指数明显增高;而两种药物干预后,72h后明显促进细胞增殖,抑制细胞凋亡。结论:金叶败毒制剂可能通过促进HCMV感染细胞的增殖,抑制感染细胞的凋亡而发挥抗病毒作用。Objective： To explore the effect of Jinyebaidu preparation on proliferation and apoptosis of human embryonic lung fibro- blast celia infected by human eytomegalovirus （ HCMV ） . Methods ： The human embryonic lung fibroblast cells were infected by HCM- VAD169, then intervened by Jinyebaidu preparation and ganciclovir; MTT method was used to detect HCMV infection and proliferative activity of human embryonic lung fibroblast cells after intervention; human embryonic lung fibroblast ceils infection, HCMV infection and apoptosis index after intervention were detected by flow eytometry. Results ： Within 48 hours after HCMV infection, there was no significant difference in cell proliferation and apoptosis index between study group and control group. Cell proliferation was inhibited and apoptosis index increased on the seventy - second hour after HCMV infection, after 72 hours, the effects were significant. Conclusion ： Jinyebaidu preparation plays an antiviral role by promoting proliferation of HCMV infectious cells and inhibiting apoptosis of infectious cells.

关 键 词：金叶败毒制剂 巨细胞病毒 增殖活性 凋亡指数 

分 类 号：R373[医药卫生—病原生物学]